Short-term effects of increased CO2, nitrate and temperature on photosynthetic activity in Ulva rigida (Chlorophyta) estimated by different pulse amplitude modulated fluorometers and oxygen evolution

Type Article
Date 2021-02
Language English
Author(s) Figueroa F L1, Bonomi-Barufi J2, Celis-Plá P S M3, 4, Nitschke U5, Arenas F6, Connan S7, 8, Abreu M H9, Malta E-J10, Conde-Álvarez R1, Chow F11, Mata M T12, Meyerhoff O13, Robledo D14, Stengel D B5
Affiliation(s) 1 : Malaga University. Institute of Blue Biotechnology and Development (IBYDA), Ecology Department, Faculty of Sciences, Campus Universitario de Teatinos s/n, Malaga, Spain
2 : Botany Departament, Federal University of Santa Catarina. Campus Trindade s/n, Florianópolis, SC, Brazil
3 : Laboratory of Costal Environmental Research,Center of Advances Studies. University of Playa Ancha. Traslaviña, Viña del Mar, Chile
4 : HUB AMBIENTAL UPLA, Vicerrectoría de Investigación, Postgrado e Innovación, Universidad de Playa Ancha, Valparaíso, Chile
5 : Botany and Plant Science, School of Natural Sciences, Ryan Institute for Environmental, Marine and Energy Research, National University of Ireland Galway, Galway, Ireland
6 : CIIMAR/CIMAR - Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Rua dos Bragas, Porto, Portugal
7 : CNRS, GEPEA, UMR6144, Boulevard de l’Université, CRTT BP 406, 44602 Saint Nazaire Cedex, France
8 : Univ Brest, CNRS, IRD, Ifremer, LEMAR, Plouzane, France
9 : ALGAplus Ltd – R&D Department, Rua António Castilho, Angeja, Portugal
10 : Centro IFAPA Agua del Pino, Crtra. El Rómpido – Punta Umbría, Cartaya (Huelva), Spain
11 : Department of Botany, University of São Paulo, Rua do Matão, São Paulo, SP, Brazil
12 : Centro de Bioinnovación Antofagasta (CBIA), Faculty of Marine Sciences and Biological Resources, Antofagasta University, Antofagasta, Chile
13 : Heinz Walz GmbH Eichenring, Effeltrich, Germany
14 : CIVESTAV-IPN, Unidad Mérida Km6 Antigua Carretera a Progreso Apartado Postal, Cordemex, Mérida, Yucatán, México
Source Journal Of Experimental Botany (0022-0957) (Oxford University Press (OUP)), 2021-02 , Vol. 72 , N. 2 , P. 491-509
DOI 10.1093/jxb/eraa473
WOS© Times Cited 7
Keyword(s) Acidification, climate change, electron transport rates, in vivo chlorophyll a fluorescence, nitrate, photosystem I and II, temperature
Abstract

Short-term effects of pCO2 (700 – 380 ppm; HC-LC) and nitrate content (50-5 βM; HN-LC) on photosynthesis, estimated by different pulse amplitude modulated (PAMs) fluorometers and by oxygen evolution, were investigated in Ulva rigida (Chlorophyta) under solar radiation (ex-situ) and in the laboratory under artificial light (in-situ). After 6-days of incubation at ambient temperature (AT), algae were subjected to a 4 oC-temperature increase (AT+4oC) for 3 d. Both in-situ and ex-situ, maximal electron transport rate (ETRmax) and in situ gross photosynthesis (GP) measured by O2 evolution presented the highest values under HCHN, and the lowest under HCLN, across all measuring systems. Maximal quantum yield (Fv/Fm), and ETRmax of PSII (ETR(II)max) and of PSI (ETR(I)max), decreased under HCLN under AT+4°C. Ex situ ETR was higher than in situ ETR. At noon, Fv/Fm decreased (indicating photoinhibition), whereas ETR(II)max and maximal non-photochemical quenching (NPQmax) increased. ETR(II)max decreased under AT+4oC in contrast to Fv/Fm, photosynthetic efficiency (αETR) and saturated irradiance (EK). Thus, U. rigida exhibited a decrease in photosynthetic production under acidification, LN levels and AT+4oC. These results emphasize the importance of studying the interactive effects between environmental parameters using in-situ vs. ex-situ conditions when aiming to evaluate the impact of global change on marine macroalgae.

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Figueroa F L, Bonomi-Barufi J, Celis-Plá P S M, Nitschke U, Arenas F, Connan S, Abreu M H, Malta E-J, Conde-Álvarez R, Chow F, Mata M T, Meyerhoff O, Robledo D, Stengel D B (2021). Short-term effects of increased CO2, nitrate and temperature on photosynthetic activity in Ulva rigida (Chlorophyta) estimated by different pulse amplitude modulated fluorometers and oxygen evolution. Journal Of Experimental Botany, 72(2), 491-509. Publisher's official version : https://doi.org/10.1093/jxb/eraa473 , Open Access version : https://archimer.ifremer.fr/doc/00655/76682/