FN Archimer Export Format
PT J
TI Deciphering the Infectious Process of Colletotrichum lupini in Lupin through Transcriptomic and Proteomic Analysis
BT 
AF Dubrulle, Guillaume
   Picot, Adeline
   Madec, Stephanie
   Corre, Erwan
   Pawtowski, Audrey
   Baroncelli, Riccardo
   Zivy, Michel
   Balliau, Thierry
   Le Floch, Gaétan
   Pensec, Flora
AS 1:1;2:1;3:2;4:3;5:1;6:4;7:5;8:5;9:1;10:1;
FF 1:;2:;3:;4:;5:;6:;7:;8:;9:;10:;
C1 Laboratoire Universitaire de Biodiversité et Écologie Microbienne, ESIAB, Université de Brest, F-29280 Plouzané
   CNRS, IRD, Ifremer, LEMAR, Université de Brest, F-29280 Plouzané, France
   Station Biologique de Roscoff, FR2424 CNRS Sorbonne Université, Place Georges Teissier, 29680 Roscoff, France
   Instituto Hispano-Luso de Investigaciones Agrarias (CIALE), University of Salamanca, Calle del Duero 12, 37185 Villamayor (Salamanca), Spain
   INRAE le Moulon, Plateforme PAPPSO, ferme du Moulon, 91190 Gif-sur-Yvette, France
C2 UBO, FRANCE
   UBO, FRANCE
   UNIV SORBONNE, FRANCE
   UNIV SALAMANCA, SPAIN
   INRAE, FRANCE
UM LEMAR
IN WOS Cotutelle UMR
   DOAJ
   copubli-france
   copubli-europe
   copubli-univ-france
IF 4.167
TC 16
UR https://archimer.ifremer.fr/doc/00656/76795/77961.pdf
   https://archimer.ifremer.fr/doc/00656/76795/77962.zip
LA English
DT Article
DE ;anthracnose disease;transcriptome;proteome;pathogenicity factors
AB The fungal phytopathogen Colletotrichum lupini is responsible for lupin anthracnose, resulting in significant yield losses worldwide. The molecular mechanisms underlying this infectious process are yet to be elucidated. This study proposes to evaluate C. lupini gene expression and protein synthesis during lupin infection, using, respectively, an RNAseq-based transcriptomic approach and a mass spectrometry-based proteomic approach. Patterns of differentially-expressed genes in planta were evaluated from 24 to 84 hours post-inoculation, and compared to in vitro cultures. A total of 897 differentially-expressed genes were identified from C. lupini during interaction with white lupin, of which 520 genes were predicted to have a putative function, including carbohydrate active enzyme, effector, protease or transporter-encoding genes, commonly described as pathogenicity factors for other Colletotrichum species during plant infection, and 377 hypothetical proteins. Simultaneously, a total of 304 proteins produced during the interaction were identified and quantified by mass spectrometry. Taken together, the results highlight that the dynamics of symptoms, gene expression and protein synthesis shared similarities to those of hemibiotrophic pathogens. In addition, a few genes with unknown or poorly-described functions were found to be specifically associated with the early or late stages of infection, suggesting that they may be of importance for pathogenicity. This study, conducted for the first time on a species belonging to the Colletotrichum acutatum species complex, presents an opportunity to deepen functional analyses of the genes involved in the pathogenicity of Colletotrichum spp. during the onset of plant infection. 
PY 2020
PD OCT
SO Microorganisms
SN 2076-2607
PU MDPI AG
VL 8
IS 10
UT 000587427600001
DI 10.3390/microorganisms8101621
ID 76795
ER

EF