FN Archimer Export Format PT J TI A trophic latitudinal gradient revealed in anchovy and sardine from the Western Mediterranean Sea using a multi-proxy approach BT AF Bachiller, Eneko Albo-Puigserver, Marta Giménez, Joan Pennino, Maria Grazia Marí-Mena, Neus Esteban, Antonio Lloret-Lloret, Elena Jadaud, Angelique Carro, Belén Bellido, José María Coll, Marta AS 1:1;2:1;3:1;4:2;5:3;6:4;7:1;8:5;9:3;10:4;11:1; FF 1:;2:;3:;4:;5:;6:;7:;8:PDG-RBE-MARBEC-LHM;9:;10:;11:; C1 Institut de Ciències del Mar (ICM-CSIC), Passeig Marítim de la Barceloneta, 37-49, 08003, Barcelona, Spain Instituto Español de Oceanografía, Centro Oceanográfico de Vigo, Subida Radio Faro, 50, 36390, Vigo, Spain AllGenetics and Biology SL, Edificio CICA, Campus de Elviña, 15008, A Coruña, Spain Instituto Español de Oceanografía, Centro Oceanográfico de Murcia, Varadero 1 Apdo 22, 30740, San Pedro del Pinatar, Murcia, Spain Marine Biodiversity, Exploitation and Conservation (MARBEC), Ifremer, University Montpellier, CNRS, IRD, Sète, France C2 ICM-CSIC, SPAIN IEO, SPAIN ALLGENETICS AND BIOLOGY SL, SPAIN IEO, SPAIN IFREMER, FRANCE SI SETE SE PDG-RBE-MARBEC-LHM UM MARBEC IN WOS Ifremer UMR DOAJ copubli-europe IF 4.379 TC 24 UR https://archimer.ifremer.fr/doc/00656/76807/77975.pdf https://archimer.ifremer.fr/doc/00656/76807/77976.docx LA English DT Article CR MEDITS MEDITS 2018 BO L'Europe AB This work combines state-of-the-art methods (DNA metabarcoding) with classic approaches (visual stomach content characterization and stable isotope analyses of nitrogen (δ15N) and carbon (δ13C)) to investigate the trophic ecology of anchovy (Engraulis encrasicolus) and sardine (Sardina pilchardus) at high taxonomic and spatial resolution in the Western Mediterranean Sea. Gut contents observed are in accordance with the dietary plasticity generally described for anchovy and sardine, suggesting a diet related to the opportunistic ingestion of available prey in a certain area and/or time. Genetic tools also showed modest inter-specific differences regarding ingested species. However, inter-specific and intra-specific differences in ingested prey frequencies and prey biomass reflected a latitudinal signal that could indicate a more effective predation on large prey like krill by anchovy versus sardine, as well as a generalized higher large prey ingestion by both species southwards. In fact, both species presented lower δ15N in the northernmost area. This latitudinal gradient indicates changes in the trophic ecology of anchovy and sardine that coincide with previously described better biological conditions for fish in the southern part of the study area as well as higher landings of both species in recent years. PY 2020 PD OCT SO Scientific Reports SN 2045-2322 PU Springer Science and Business Media LLC VL 10 IS 1 UT 000585186800005 DI 10.1038/s41598-020-74602-y ID 76807 ER EF