TY - JOUR T1 - An improved whole life cycle culture protocol for the hydrozoan genetic model Clytia hemisphaerica A1 - Lechable,Marion A1 - Jan,Anne-Cecile A1 - Duchene,Axel A1 - Uveira,Julie A1 - Weissbourd,Brandon A1 - Gissat,Loann A1 - Collet,Sophie A1 - Gilletta,Laurent A1 - Chevalier,Sandra A1 - Leclère,Lucas A1 - Peron,Sophie A1 - Barreau,Carine A1 - Lasbleiz,Régis A1 - Houliston,Evelyn A1 - Momose,Tsuyoshi AD - Sorbonne Universités, CNRS, Institut de la Mer de Villefranche, Laboratoire de Biologie du Développement de Villefranche-sur-Mer (LBDV), 06230 Villefranche-sur-Mer, France AD - Sorbonne Universités, Institut de la mer de Villefranche, FR3761, Service Aquariologie, 06230 Villefranche-sur-Mer, France AD - California Institute of Technology, Division of Biology, and the Howard Hughes Medical Institute, 1200 E California Blvd, Pasadena CA 91125, USA UR - https://archimer.ifremer.fr/doc/00656/76820/ DO - 10.1242/bio.051268 KW - Animal culture KW - Cnidarian KW - Developmental biology KW - Genetics KW - Jellyfish N2 - The jellyfish species Clytia hemisphaerica (Cnidaria, Hydrozoa) has emerged as a new experimental model animal in the last decade. Favorable characteristics include a fully transparent body suitable for microscopy, daily gamete production and a relatively short life cycle. Furthermore, whole genome sequence assembly and efficient gene editing techniques using CRISPR/Cas9 have opened new possibilities for genetic studies. The quasi-immortal vegetatively-growing polyp colony stage provides a practical means to maintain mutant strains. In the context of developing Clytia as a genetic model, we report here an improved whole life cycle culture method including an aquarium tank system designed for culture of the tiny jellyfish form. We have compared different feeding regimes using Artemia larvae as food and demonstrate that the stage-dependent feeding control is the key for rapid and reliable medusa and polyp rearing. Metamorphosis of the planula larvae into a polyp colony can be induced efficiently using a new synthetic peptide. The optimized procedures detailed here make it practical to generate genetically modified Clytia strains and to maintain their whole life cycle in the laboratory. Y1 - 2020/11 PB - The Company of Biologists JF - Biology Open SN - 2046-6390 VL - 9 IS - 11 ID - 76820 ER -