FN Archimer Export Format
PT J
TI An improved whole life cycle culture protocol for the hydrozoan genetic model   Clytia hemisphaerica
BT 
AF Lechable, Marion
   Jan, Anne-Cecile
   Duchene, Axel
   Uveira, Julie
   Weissbourd, Brandon
   Gissat, Loann
   Collet, Sophie
   Gilletta, Laurent
   Chevalier, Sandra
   Leclère, Lucas
   Peron, Sophie
   Barreau, Carine
   Lasbleiz, Régis
   Houliston, Evelyn
   Momose, Tsuyoshi
AS 1:1;2:1,2;3:2;4:1;5:3;6:2;7:2;8:1;9:1;10:1;11:1;12:1;13:2;14:1;15:1;
FF 1:;2:;3:;4:;5:;6:;7:;8:;9:;10:;11:;12:;13:;14:;15:;
C1 Sorbonne Universités, CNRS, Institut de la Mer de Villefranche, Laboratoire de Biologie du Développement de Villefranche-sur-Mer (LBDV), 06230 Villefranche-sur-Mer, France
   Sorbonne Universités, Institut de la mer de Villefranche, FR3761, Service Aquariologie, 06230 Villefranche-sur-Mer, France
   California Institute of Technology, Division of Biology, and the Howard Hughes Medical Institute, 1200 E California Blvd, Pasadena CA 91125, USA
C2 UNIV SORBONNE, FRANCE
   UNIV SORBONNE, FRANCE
   CALTECH, USA
IN DOAJ
IF 2.422
TC 18
UR https://archimer.ifremer.fr/doc/00656/76820/77996.pdf
   https://archimer.ifremer.fr/doc/00656/76820/77997.pdf
LA English
DT Article
DE ;Animal culture;Cnidarian;Developmental biology;Genetics;Jellyfish
AB The jellyfish species Clytia hemisphaerica (Cnidaria, Hydrozoa) has emerged as a new experimental model animal in the last decade. Favorable characteristics include a fully transparent body suitable for microscopy, daily gamete production and a relatively short life cycle. Furthermore, whole genome sequence assembly and efficient gene editing techniques using CRISPR/Cas9 have opened new possibilities for genetic studies. The quasi-immortal vegetatively-growing polyp colony stage provides a practical means to maintain mutant strains. In the context of developing Clytia as a genetic model, we report here an improved whole life cycle culture method including an aquarium tank system designed for culture of the tiny jellyfish form. We have compared different feeding regimes using Artemia larvae as food and demonstrate that the stage-dependent feeding control is the key for rapid and reliable medusa and polyp rearing. Metamorphosis of the planula larvae into a polyp colony can be induced efficiently using a new synthetic peptide. The optimized procedures detailed here make it practical to generate genetically modified Clytia strains and to maintain their whole life cycle in the laboratory. 
PY 2020
PD NOV
SO Biology Open
SN 2046-6390
PU The Company of Biologists
VL 9
IS 11
UT 000588344300001
DI 10.1242/bio.051268
ID 76820
ER

EF