FN Archimer Export Format
PT J
TI Transcriptomic analysis of polyketide synthases in a highly ciguatoxic dinoflagellate, Gambierdiscus polynesiensis and low toxicity Gambierdiscus pacificus, from French Polynesia
BT 
AF VAN DOLAH, Frances M.
   MOREY, Jeanine S.
   MILNE, Shard
   UNG, Andre
   ANDERSON, Paul E.
   CHINAIN, Mireille
AS 1:1,4;2:1,5;3:2,6;4:3;5:2,7;6:3;
FF 1:;2:;3:;4:;5:;6:;
C1 Hollings Marine Lab, Marine Genom Core, Charleston, SC 29412 USA.
   Coll Charleston, Dept Comp Sci, Charleston Computat Genom Grp, Charleston, SC 29401 USA.
   Inst Louis Malarde UMR 241 EIO, Lab Biotoxines Marines, Tahiti, French Polynesi, France.
   Univ Charleston, Grad Program Marine Biol, Charleston, SC 29424 USA.
   Natl Marine Mammal Fdn, Johns Isl, SC USA.
   Univ Washington, Sch Environm & Forest Sci, Seattle, WA 98195 USA.
   Calif Polytech State Univ San Luis Obispo, Dept Comp Sci & Software Engn, San Luis Obispo, CA 93407 USA.
C2 HOLLINGS MARINE LAB, USA
   COLL CHARLESTON, USA
   INST LOUIS MALARDE, FRANCE
   UNIV CHARLESTON, USA
   NATL MARINE MAMMAL FDN, USA
   UNIV WASHINGTON, USA
   UNIV CALIF POLYTECH, USA
UM EIO
IN WOS Cotutelle UMR
   DOAJ
   copubli-int-hors-europe
IF 3.24
TC 13
UR https://archimer.ifremer.fr/doc/00657/76863/78096.pdf
   https://archimer.ifremer.fr/doc/00657/76863/78097.pptx
   https://archimer.ifremer.fr/doc/00657/76863/78098.pptx
   https://archimer.ifremer.fr/doc/00657/76863/78099.xlsx
   https://archimer.ifremer.fr/doc/00657/76863/78100.xlsx
   https://archimer.ifremer.fr/doc/00657/76863/78101.xlsx
   https://archimer.ifremer.fr/doc/00657/76863/78102.xlsx
   https://archimer.ifremer.fr/doc/00657/76863/78103.xlsx
LA English
DT Article
AB Marine dinoflagellates produce a diversity of polyketide toxins that are accumulated in marine food webs and are responsible for a variety of seafood poisonings. Reef-associated dinoflagellates of the genus Gambierdiscus produce toxins responsible for ciguatera poisoning (CP), which causes over 50,000 cases of illness annually worldwide. The biosynthetic machinery for dinoflagellate polyketides remains poorly understood. Recent transcriptomic and genomic sequencing projects have revealed the presence of Type I modular polyketide synthases in dinoflagellates, as well as a plethora of single domain transcripts with Type I sequence homology. The current transcriptome analysis compares polyketide synthase (PKS) gene transcripts expressed in two species of Gambierdiscus from French Polynesia: a highly toxic ciguatoxin producer, G. polynesiensis, versus a non-ciguatoxic species G. pacificus, each assembled from approximately 180 million Illumina 125 nt reads using Trinity, and compares their PKS content with previously published data from other Gambierdiscus species and more distantly related dinoflagellates. Both modular and single-domain PKS transcripts were present. Single domain beta-ketoacyl synthase (KS) transcripts were highly amplified in both species (98 in G. polynesiensis, 99 in G. pacificus), with smaller numbers of standalone acyl transferase (AT), ketoacyl reductase (KR), dehydratase (DH), enoyl reductase (ER), and thioesterase (TE) domains. G. polynesiensis expressed both a larger number of multidomain PKSs, and larger numbers of modules per transcript, than the non-ciguatoxic G. pacificus. The largest PKS transcript in G. polynesiensis encoded a 10,516 aa, 7 module protein, predicted to synthesize part of the polyether backbone. Transcripts and gene models representing portions of this PKS are present in other species, suggesting that its function may be performed in those species by multiple interacting proteins. This study contributes to the building consensus that dinoflagellates utilize a combination of Type I modular and single domain PKS proteins, in an as yet undefined manner, to synthesize polyketides.
PY 2020
PD APR
SO Plos One
SN 1932-6203
PU Public Library Science
VL 15
IS 4
UT 000536003500034
DI 10.1371/journal.pone.0231400
ID 76863
ER

EF