Strong constitutive expression divergence among strains but no evidence of differential expression associated with sexual reproduction in Alexandrium minutum

Type Article
Date 2020-12
Language English
Author(s) Seveno Julie1, 2, Even Yasmine3, Le Gac MickaelORCID1
Affiliation(s) 1 : Ifremer, DYNECO PELAGOS, 29280 Plouzané, France
2 : Laboratoire Mer Molécule Santé, Le Mans Université, 72000 Le Mans, France
3 : Laboratoire des Sciences de l'Environnement Marin, UMR 6539 CNRS/UBO – Institut Universitaire Européen de la Mer, Technopôle Brest-Iroise, 29280 Plouzané, France
Source Harmful Algae (1568-9883) (Elsevier BV), 2020-12 , Vol. 100 , P. 101940 (12p.)
DOI 10.1016/j.hal.2020.101940
Keyword(s) Sexual reproduction, Alexandrium minutum, Gene expression, Mating
Abstract

Sexual reproduction remains poorly characterized in dinoflagellates. This is especially the case at the molecular level. Here crossing experiments were performed among strains of the toxic dinoflagellate Alexandrium minutum belonging to two genetically divergent groups. Gene expression was compared between sexually compatible and incompatible crosses at the time of gamete fusion and resting cyst (~zygote) formation. Not a single transcript was identified as differentially expressed between compatible and incompatible crosses at these two crucial time points of the dinoflagellate life cycle. However, several thousands of transcripts displayed constitutive expression differences between strains. This was especially the case between the strains belonging to the genetically divergent groups. A few hundreds of transcripts were also identified as differentially expressed between strains belonging to opposite mating types. Some of these transcripts displayed homology with the SxtA protein, known to be involved in saxitoxin production in cyanobacteria, as well as with proteins potentially involved in mating in fungi.

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