FN Archimer Export Format PT J TI Strong constitutive expression divergence among strains but no evidence of differential expression associated with sexual reproduction in Alexandrium minutum BT AF Seveno, Julie Even, Yasmine Le Gac, Mickael AS 1:1,2;2:3;3:1; FF 1:;2:;3:PDG-ODE-DYNECO-PELAGOS; C1 Ifremer, DYNECO PELAGOS, 29280 Plouzané, France Laboratoire Mer Molécule Santé, Le Mans Université, 72000 Le Mans, France Laboratoire des Sciences de l'Environnement Marin, UMR 6539 CNRS/UBO – Institut Universitaire Européen de la Mer, Technopôle Brest-Iroise, 29280 Plouzané, France C2 IFREMER, FRANCE UNIV LE MANS, FRANCE UBO, FRANCE SI BREST SE PDG-ODE-DYNECO-PELAGOS UM LEMAR IN WOS Ifremer UPR WOS Cotutelle UMR copubli-france copubli-univ-france IF 4.273 TC 1 UR https://archimer.ifremer.fr/doc/00658/77022/78835.pdf LA English DT Article DE ;Sexual reproduction;Alexandrium minutum;Gene expression;Mating AB Sexual reproduction remains poorly characterized in dinoflagellates. This is especially the case at the molecular level. Here crossing experiments were performed among strains of the toxic dinoflagellate Alexandrium minutum belonging to two genetically divergent groups. Gene expression was compared between sexually compatible and incompatible crosses at the time of gamete fusion and resting cyst (~zygote) formation. Not a single transcript was identified as differentially expressed between compatible and incompatible crosses at these two crucial time points of the dinoflagellate life cycle. However, several thousands of transcripts displayed constitutive expression differences between strains. This was especially the case between the strains belonging to the genetically divergent groups. A few hundreds of transcripts were also identified as differentially expressed between strains belonging to opposite mating types. Some of these transcripts displayed homology with the SxtA protein, known to be involved in saxitoxin production in cyanobacteria, as well as with proteins potentially involved in mating in fungi. PY 2020 PD DEC SO Harmful Algae SN 1568-9883 PU Elsevier BV VL 100 UT 000599869000004 DI 10.1016/j.hal.2020.101940 ID 77022 ER EF