FN Archimer Export Format
PT J
TI Potential for Genetic Improvement of Resistance to Perkinsus olseni in the Manila Clam, Ruditapes philippinarum, Using DNA Parentage Assignment and Mass Spawning
BT 
AF Smits, Morgan
   Enez, Florian
   Ferraresso, Serena
   Dalla Rovere, Giulia
   Vetois, Emilie
   Auvray, Jean-François
   Genestout, Lucie
   Mahla, Rachid
   Arcangeli, Giuseppe
   Paillard, Christine
   Haffray, Pierrick
   Bargelloni, Luca
AS 1:1;2:2;3:1;4:1;5:3;6:3;7:4;8:4;9:5;10:6;11:2;12:1;
FF 1:;2:;3:;4:;5:;6:;7:;8:;9:;10:;11:;12:;
C1 Department of Comparative Biomedicine and Food Science (BCA), University of Padova, Legnaro, Italy
   Syndicat des Sélectionneurs Avicoles et Aquacoles Français (SYSAAF), Laboratoire de Physiologie et Génomique des Poissons (LPGP), Campus de Beaulieu, Rennes, France
   Société Atlantique de Mariculture (SATMAR), Gatteville-Phare, France
   Labogena, Domaine de Vilvert, Jouy en Josas, France
   National Reference Centre for Fish, Crustacean and Mollusc Pathology, Italian Health Authority and Research Organization for Animal Health and Food Safety (IZSVe), Legnaro, Italy
   Laboratory of Marine Environmental Sciences (LEMAR), Institut Universitaire Européen de la Mer, Plouzané, France
C2 UNIV PADOVA, ITALY
   SYSAAF, FRANCE
   SATMAR, FRANCE
   LABOGENA, FRANCE
   IZSVE, ITALY
   CNRS, FRANCE
UM LEMAR
IN WOS Cotutelle UMR
   DOAJ
   copubli-france
   copubli-europe
IF 2.029
TC 7
UR https://archimer.ifremer.fr/doc/00658/77031/78319.pdf
   https://archimer.ifremer.fr/doc/00658/77031/78320.docx
LA English
DT Article
DE ;Manila clam (Ruditapes philippinarum);Perkinsus olseni infection;disease resistance;genetic selection;genetic parameter estimation
AB The Manila clam Ruditapes philippinarum, a major cultured shellfish species, is threatened by infection with the microparasite Perkinsus olseni, whose prevalence increases with high water temperatures. Under the current trend of climate change, the already severe effects of this parasitic infection might rapidly increase the frequency of mass mortality events. Treating infectious diseases in bivalves is notoriously problematic, therefore selective breeding for resistance represents a key strategy for mitigating the negative impact of pathogens. A crucial step in initiating selective breeding is the estimation of genetic parameters for traits of interest, which relies on the ability to record parentage and accurate phenotypes in a large number of individuals. Here, to estimate the heritability of resistance against P. olseni, a field experiment mirroring conditions in industrial clam production was set up, a genomic tool was developed for parentage assignment, and parasite load was determined through quantitative PCR. A mixed-family cohort of potentially 1,479 clam families was produced in a hatchery by mass spawning of 53 dams and 57 sires. The progenies were seeded in a commercial clam production area in the Venice lagoon, Italy, where high prevalence of P. olseni had previously been reported. Growth and parasite load were monitored every month and, after 1 year, more than 1,000 individuals were collected for DNA samples and phenotype recording. A pooled sequencing approach was carried out using DNA samples from the hatchery broodstock and from a Venice lagoon clam population, providing candidate markers used to develop a 245-SNP panel. Parentage assignment for 246 F1 individuals showed sire and dam representation were high (75 and 85%, respectively), indicating a very limited risk of inbreeding. Moderate heritability (0.23 ± 0.11–0.35 ± 0.13) was estimated for growth traits (shell length, shell weight, total weight), while parasite load showed high heritability, estimated at 0.51 ± 0.20. No significant genetic correlations were found between growth-associated traits and parasite load. Overall, the preliminary results provided by this study show high potential for selecting clams resistant to parasite load. Breeding for resistance may help limit the negative effects of climate change on clam production, as the prevalence of the parasite is predicted to increase under a future scenario of higher temperatures. Finally, the limited genetic correlation between resistance and growth suggests that breeding programs could incorporate dual selection without negative interactions. 
PY 2020
PD OCT
SO Frontiers In Veterinary Science
SN 2297-1769
PU Frontiers Media SA
VL 7
UT 000585190900001
DI 10.3389/fvets.2020.579840
ID 77031
ER

EF