FN Archimer Export Format
PT J
TI Cosmopolitan Distribution of Endozoicomonas-Like Organisms and Other Intracellular Microcolonies of Bacteria Causing Infection in Marine Mollusks
BT 
AF Cano, Irene
   Ryder, David
   Webb, Steve C.
   Jones, Brian J.
   Brosnahan, Cara L.
   Carrasco, Noelia
   Bodinier, Barbara
   Furones, Dolors
   Pretto, Tobia
   Carella, Francesca
   Chollet, Bruno
   Arzul, Isabelle
   Cheslett, Deborah
   Collins, Evelyn
   Lohrmann, Karin B.
   Valdivia, Ana L.
   Ward, Georgia
   Carballal, María J.
   Villalba, Antonio
   Marigómez, Ionan
   Mortensen, Stein
   Christison, Kevin
   Kevin, Wakeman C.
   Bustos, Eduardo
   Christie, Lyndsay
   Green, Matthew
   Feist, Stephen W.
AS 1:1;2:1;3:2;4:3;5:3;6:4;7:4;8:4;9:5;10:6;11:7;12:7;13:8;14:9;15:9;16:10;17:10;18:10,11,12;19:12;20:13;21:14;22:15;23:16;24:17;25:1;26:1;27:1;
FF 1:;2:;3:;4:;5:;6:;7:;8:;9:;10:;11:PDG-RBE-SGMM-LGPMM;12:PDG-RBE-SGMM-LGPMM;13:;14:;15:;16:;17:;18:;19:;20:;21:;22:;23:;24:;25:;26:;27:;
C1 International Centre of Excellence for Aquatic Animal Health, Cefas Weymouth Laboratory, Weymouth, United Kingdom
   Cawthron Institute, Nelson, New Zealand
   Animal Health Laboratory, Ministry for Primary Industries, Upper Hutt, New Zealand
   Institut de Recerca i Tecnologia Agroalimentaries (IRTA), Sant Carles de la Ràpita, Tarragona, Spain
   Istituto Zooprofilattico Sperimentale delle Venezie, Legnaro, Italy
   Department of Biology, University of Naples Federico II, Naples, Italy
   SG2M-LGPMM, Laboratoire De Génétique Et Pathologie Des Mollusques Marins, Ifremer, La Tremblade, France
   Marine Institute, Galway, Ireland
   Departamento de Biología Marina, Facultad de Ciencias del Mar, Universidad Católica del Norte, Centro Innovación Acuícola Aquapacífico, Coquimbo, Chile
   Life Sciences Department, Natural History Museum, London, United Kingdom
   Centro de Investigacións Mariñas, Consellería do Mar da Xunta de Galicia, Vilanova de Arousa, Spain
   Centro de Investigacións Mariñas, Consellería do Mar da Xunta de Galicia, Vilanova de Arousa, Spain
   Research Centre for Experimental Marine Biology and Biotechnology (PIE), University of the Basque Country (UPV/EHU), Plentzia, Spain
   Institute of Marine Research, Bergen, Norway
   Department of Environment, Forestry and Fisheries, Cape Town, South Africa
   Institute for International Collaboration, Hokkaido University, Sapporo, Japan
   Centro Acuícola Pesquero de Investigación Aplicada (CAPIA), Universidad Santo Tomás, Sede Puerto Montt, Chile
C2 CEFAS, UK
   INST CAWTHRON, NEW ZEALAND
   MINIST PRIMARY INDUST, NEW ZEALAND
   IRTA, SPAIN
   IZSVE, ITALY
   UNIV NAPLES, ITALY
   IFREMER, FRANCE
   MARINE INST, IRELAND
   UNIV CATOLICA NORTE, CHILE
   NHM, UK
   CIMA, SPAIN
   CIMA, SPAIN
   UNIV PAIS VASCO EHU, SPAIN
   IMR (BERGEN), NORWAY
   DEPART ENVIRONM FORESTRY FISHERIES, SOUTH AFRICA
   UNIV HOKKAIDO, JAPAN
   UNIV SANTO TOMAS, CHILE
SI LA TREMBLADE
SE PDG-RBE-SGMM-LGPMM
IN WOS Ifremer UPR
   DOAJ
   copubli-europe
   copubli-int-hors-europe
   copubli-sud
IF 3.941
TC 18
UR https://archimer.ifremer.fr/doc/00658/77032/78322.pdf
   https://archimer.ifremer.fr/doc/00658/77032/78325.docx
   https://archimer.ifremer.fr/doc/00658/77032/78327.xlsx
LA English
DT Article
DE ;mollusk;OTU;Endozoicomonas;Chlamydiae;Mycoplasma;Francisella;Lucinoma;Ridgeia
AB Intracellular microcolonies of bacteria (IMC), in some cases developing large extracellular cysts (bacterial aggregates), infecting primarily gill and digestive gland, have been historically reported in a wide diversity of economically important mollusk species worldwide, sometimes associated with severe lesions and mass mortality events. As an effort to characterize those organisms, traditionally named as Rickettsia or Chlamydia-like organisms, 1950 specimens comprising 22 mollusk species were collected over 10 countries and after histology examination, a selection of 99 samples involving 20 species were subjected to 16S rRNA gene amplicon sequencing. Phylogenetic analysis showed Endozoicomonadaceae sequences in all the mollusk species analyzed. Geographical differences in the distribution of Operational Taxonomic Units (OTUs) and a particular OTU associated with pathology in king scallop (OTU_2) were observed. The presence of Endozoicomonadaceae sequences in the IMC was visually confirmed by in situ hybridization (ISH) in eight selected samples. Sequencing data also indicated other symbiotic bacteria. Subsequent phylogenetic analysis of those OTUs revealed a novel microbial diversity associated with molluskan IMC infection distributed among different taxa, including the phylum Spirochetes, the families Anaplasmataceae and Simkaniaceae, the genera Mycoplasma and Francisella, and sulfur-oxidizing endosymbionts. Sequences like Francisella halioticida/philomiragia and Candidatus Brownia rhizoecola were also obtained, however, in the absence of ISH studies, the association between those organisms and the IMCs were not confirmed. The sequences identified in this study will allow for further molecular characterization of the microbial community associated with IMC infection in marine mollusks and their correlation with severity of the lesions to clarify their role as endosymbionts, commensals or true pathogens. 
PY 2020
PD OCT
SO Frontiers In Microbiology
SN 1664-302X
PU Frontiers Media SA
VL 11
UT 000588677100001
DI 10.3389/fmicb.2020.577481
ID 77032
ER

EF