FN Archimer Export Format
PT J
TI A rapid diagnostic multiplex PCR approach for xenomonitoring of human and animal schistosomiasis in a 'One Health' context
BT 
AF SCHOLS, Ruben
   CAROLUS, Hans
   HAMMOUD, Cyril
   MULERO, Stephen
   MUDAVANHU, Aspire
   HUYSE, Tine
AS 1:1;2:1;3:2,3;4:4;5:5;6:1,2;
FF 1:;2:;3:;4:;5:;6:;
C1 Univ Leuven, Lab Biodivers & Evolutionary Genom, B-3000 Leuven, Belgium.
   Royal Museum Cent Africa, Dept Biol, Leuvensesteenweg 13, B-3080 Tervuren, Belgium.
   Univ Ghent, Limnol Res Unit, KL Ledeganckstr 35, B-9000 Ghent, Belgium.
   Univ Perpignan, CNRS, Lab Host Pathogen Environm Interact, IHPE UMR 5244, Via Domitia, F-66100 Perpignan, France.
   Univ Zimbabwe, Dept Biol Sci, Harare, Zimbabwe.
C2 UNIV LEUVEN, BELGIUM
   ROYAL MUSEUM CENT AFRICA, BELGIUM
   UNIV GHENT, BELGIUM
   UNIV PERPIGNAN, FRANCE
   UNIV ZIMBABWE, ZIMBABWE
UM IHPE
IN WOS Cotutelle UMR
   copubli-europe
   copubli-int-hors-europe
   copubli-sud
IF 1.868
TC 22
UR https://archimer.ifremer.fr/doc/00659/77115/86967.pdf
LA English
DT Article
DE ;gastropod-borne disease;One Health;rapid diagnostic multiplex PCR;Schistosoma;transmission monitoring;Trematoda
AB Studying the epidemiology of schistosomiasis-the most prevalent gastropod-borne human disease and an economic burden for the livestock industry-relies on adequate monitoring tools. Here we describe a molecular assay for detecting human and animal African schistosome species in their planorbid gastropod host (xenomonitoring) using a two-step approach. First, schistosome infections are detected and discriminated from other trematode infections using a multiplex polymerase chain reaction (PCR) that includes a trematode-specific marker (in 18S rDNA), a Schistosoma genus-specific marker (in internal transcribed spacer 2 [ITS2]) and a general gastropod marker (in 18S rDNA) as an internal control. Upon Schistosoma sp. detection, a second multiplex PCR is performed to discriminate among Schistosoma haematobium, Schistosoma mansoni, Schistosoma mattheei and Schistosoma bovis/Schistosoma curassoni/Schistosoma guineensis using markers of differential lengths in the cytochrome c oxidase subunit 1 (COX1) gene. The specificity of these assays was validated with adult worms, naturally infected gastropods and human urine and stool samples. Sensitivity was tested on experimentally infected snail specimens that were sacrificed 10 and 40 days post-infection in order to mimic a natural prepatent and mature infection, respectively. The assay provides a diagnostic tool to support the xenomonitoring of planorbid gastropods for trematode infections in a One Health context, with a focus on the transmission monitoring of schistosomiasis.
PY 2019
PD NOV
SO Transactions Of The Royal Society Of Tropical Medicine And Hygiene
SN 0035-9203
PU Oxford Univ Press
VL 113
IS 11
UT 000498170700010
BP 722
EP 729
DI 10.1093/trstmh/trz067
ID 77115
ER

EF