Chemodiversity of Calophyllum inophyllum L. oil bioactive components related to their specific geographical distribution in the South Pacific region

Type Article
Date 2019-05
Language English
Author(s) Ginigini Joape1, Lecellier Gael J.2, Nicolas Mael3, Nour Mohammed3, Hnawia Edouard3, Lebouvier Nicolas3, Herbette Gaetan4, Lockhart Peter5, Raharivelomanana Phila6
Affiliation(s) 1 : Univ South Pacific, Pacific Nat Prod Res Ctr, Inst Appl Sci, Suva, Fiji.
2 : Univ Versailles St Quentin En Yvelines, Dept Biol, Versailles, France.
3 : Univ Caledonia, ISEA EA7484, Noumea, New Caledonia.
4 : Aix Marseille Univ, FSCM, Cent Marseille, CNRS,Spectropole, Campus St Jerome, Marseille, France.
5 : Massey Univ, Sch Fundamental Sci, Palmerston North, New Zealand.
6 : Univ Polynesie Francaise, EIO UMR241, Tahiti, French Polynesi, France.
Source Peerj (2167-8359) (Peerj Inc), 2019-05 , Vol. 7 , P. e6896 (21p.=
DOI 10.7717/peerj.6896
WOS© Times Cited 2
Keyword(s) Calophyllum inophyllum, Chemodiversity, South Pacific, Neoflavonoids, Oil, Biodiversity
Abstract

Background: Different parts of the tree Calophyllum inophyllum L. (nuts, leaves, roots, bark, fruits, nut oil and resin) are used as traditional medicines and cosmetics in most of the Pacific Islands. The oil efficiency as a natural cure and in traditional cosmetics has been largely described throughout the South Pacific, which led us to investigate C. inophyllum's chemical and genetic diversity. A correlative study of the nut resin and leaf DNA from three distinct archipelagos in the South Pacific was carried out in order to identify diversity patterns in C. inophyllum across the South Pacific. Methods: Calophyllum inophyllum plants were sampled from French Polynesia, New Caledonia and Fiji. We extracted tamanu oil (nut oil) resin for chemo-diversity studies and sampled leaf tissues for genetic studies. We applied an analysis method designed for small quantities (at a microscale level), and used High Performance Liquid Chromatography (HPLC) to establish the chemo-diversity of tamanu oil resin. In-house standards were co-eluted for qualitative determination. Genetic diversity was assessed using chloroplast barcoding markers (the Acetyl-CoA carboxylase (accD) gene and the psaA-ycf3 intergenic spacer region). Results: Our HPLC analysis revealed 11 previously known tamanu oil constituents, with variability among plant samples. We also isolated and characterized two new neoflavonoids from tamanu oil resin namely, tamanolide E1 and E2 which are diastereoisomers. Although genetic analysis revealed low genetic variation, our multivariate analysis (PCA) of the tamanu oil resin chemical profiles revealed differentiation among geographic regions. Conclusion: We showed here that chromatographic analysis using formalized in-house standards of oil resin compounds for co-elution studies against oil resin samples could identify patterns of variation among samples of C. inophyllum, and discriminate samples from different geographical origins.

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Ginigini Joape, Lecellier Gael J., Nicolas Mael, Nour Mohammed, Hnawia Edouard, Lebouvier Nicolas, Herbette Gaetan, Lockhart Peter, Raharivelomanana Phila (2019). Chemodiversity of Calophyllum inophyllum L. oil bioactive components related to their specific geographical distribution in the South Pacific region. Peerj, 7, e6896 (21p.=. Publisher's official version : https://doi.org/10.7717/peerj.6896 , Open Access version : https://archimer.ifremer.fr/doc/00659/77126/