FN Archimer Export Format PT J TI R. nukuhivensis acts by reinforcing skin barrier function, boosting skin immunity and by inhibiting IL-22 induced keratinocyte hyperproliferation BT AF ABDALLAH, Florence LECELLIER, Gael RAHARIVELOMANANA, Phila PICHON, Chantal AS 1:1;2:2;3:3;4:1,4; FF 1:;2:;3:;4:; C1 CNRS, Ctr Biophys Mol, UPR4301, F-45071 Orleans, France. Univ Paris Saclay UVSQ, 55 Ave Paris, F-78000 Versailles, France. Univ Polynesie Francaise, UMR 241 EIO, F-98702 Tahiti, French Polynesi, France. Univ Orleans, Coll Sci & Tech, F-45100 Orleans, France. C2 CNRS, FRANCE UNIV VERSAILLES ST QUENTIN EN YVELINES, FRANCE UNIV POLYNESIE FRANCAISE, FRANCE UNIV ORLEANS, FRANCE UM EIO IN WOS Cotutelle UMR DOAJ copubli-france copubli-univ-france IF 3.998 TC 4 UR https://archimer.ifremer.fr/doc/00659/77130/78499.pdf https://archimer.ifremer.fr/doc/00659/77130/78500.pdf LA English DT Article AB Rauvolfia nukuhivensis is a well-known plant used for its wide range of beneficial effects in Marquesas islands. It is made up of diverse indole alkaloids and is used as traditional medicine for skin application. The actual mechanism behind the virtue of this plant is still unknown. Hence, in this study we aimed at deciphering the impact of R. nukuhivensis on skin immune system in context of (1) homeostasis, (2) pathogen infection and (3) inflammation. Here we show that R. nukuhivensis enhances cellular metabolic activity and wound healing without inducing cellular stress or disturbing cellular homeostasis. It reinforces the epithelial barrier by up-regulating hBD-1. Nevertheless, in pathogenic stress, R. nukuhivensis acts by preparing the immune system to be reactive and effective directly. Indeed, it enhances the innate immune response by increasing pathogens sensors such as TLR5. Finally, R. nukuhivensis blocks IL-22 induced hyperproliferation via PTEN and Filaggrin up-regulation as well as BCL-2 downregulation. In conclusion, this study provides evidence on the several cutaneous application potentials of R. nukuhivensis such as boosting the immune response or in restoring the integrity of the epithelial barrier. PY 2019 PD MAR SO Scientific Reports SN 2045-2322 PU Nature Publishing Group VL 9 UT 000460754600023 DI 10.1038/s41598-019-39831-w ID 77130 ER EF