FN Archimer Export Format
PT J
TI Summer bloom of Vulcanodinium rugosum in Cienfuegos Bay (Cuba) associated to dermatitis in swimmers
BT 
AF Moreira-González, Angel R.
   Comas-González, Augusto
   Valle-Pombrol, Aimee
   Seisdedo-Losa, Mabel
   Hernández-Leyva, Olidia
   Fernandes, Luciano F.
   Chomérat, Nicolas
   Bilien, Gwenael
   Hervé, Fabienne
   Rovillon, Georges-Augustin
   Hess, Philipp
   Alonso-Hernández, Carlos M.
   Mafra, Luiz L.
AS 1:1,2;2:1;3:1;4:1;5:3;6:4;7:5;8:5;9:6;10:6;11:6;12:1;13:2;
FF 1:;2:;3:;4:;5:;6:;7:PDG-ODE-LITTORAL-LERBO;8:PDG-ODE-LITTORAL-LERBO;9:PDG-ODE-DYNECO-PHYC;10:PDG-ODE-DYNECO-PHYC;11:PDG-ODE-DYNECO-PHYC;12:;13:;
C1 Centro de Estudios Ambientales de Cienfuegos (CEAC), AP. 5, Ciudad Nuclear, CP 59350, Cienfuegos, Cuba
   Centro de Estudos do Mar, Universidade Federal do Paraná, P.O. Box 61, Av. Beira Mar, s/n, Pontal do Paraná, Paraná 83255-976, Brazil
   Centro Provincial de Higiene, Epidemiología y Microbiología de Cienfuegos, 13 Calzada de Máximo Gómez, Cienfuegos 55100, Cuba
   Departamento de Botânica, Universidade Federal do Paraná, Centro Politécnico, R. Elétrica, 540, Curitiba, Paraná 82590-300, Brazil
   IFREMER, Laboratory of Environment and Resources Western Brittany, Coastal Research Unit, Quai de la Croix, 29900 Concarneau Cedex, France
   IFREMER, DYNECO, Laboratoire Phycotoxines, Rue de l'Île d'Yeu, 44311 Nantes Cedex 03, France
C2 CEAC, CUBA
   UNIV FED PARANA, BRAZIL
   CTR PROV HIGIENE EPIDEMIOL MICROBIOL CIENFUEGOS, CUBA
   UNIV FED PARANA, BRAZIL
   IFREMER, FRANCE
   IFREMER, FRANCE
SI CONCARNEAU
   NANTES
SE PDG-ODE-LITTORAL-LERBO
   PDG-ODE-DYNECO-PHYC
IN WOS Ifremer UPR
   copubli-int-hors-europe
   copubli-sud
IF 10.754
TC 19
UR https://archimer.ifremer.fr/doc/00659/77142/78467.pdf
LA English
DT Article
DE ;Harmful algal bloom;Human health;Skin irritation;Emergent toxin
AB The marine dinoflagellate Vulcanodinium rugosum produces powerful paralyzing and cytotoxic compounds named pinnatoxins (PnTX) and portimines. Even though, no related human intoxication episodes following direct exposure in seawater or the ingestion of contaminated seafood have been documented so far. This study aimed at investigating a dinoflagellate bloom linked to acute dermatitis cases in two recreational beaches in Cienfuegos Bay, Cuba. We used epidemiological and clinical data from 60 dermatitis cases consisting of individuals in close contact with the bloom. Seawater physical-chemical properties were described, and the microorganism causing the bloom was identified by means of light and scanning electron microscopy. Morphological identification was confirmed genetically by sequencing the internal transcribed spacers ITS1 and ITS2, and the 5.8S rDNA region. Toxic compounds were identified from a bloom extract using liquid chromatography (LC) coupled to high-resolution mass spectrometry (HRMS), and their concentrations were estimated based on low-resolution tandem mass spectrometry (LC-MS/MS). Sixty people who had prolonged contact with the dinoflagellate bloom suffered acute dermal irritation. Most patients (79.2%) were children and had to be treated with antibiotics; some required >5-day hospitalization. Combined morphological and genetic characters indicated V. rugosum as the causative agent of the bloom. rDNA sequences of the V. rugosum genotype found in the bloom aligned with others from Asia, including material found in the ballast tank of a ship in Florida. The predominant toxins in the bloom were portimine, PnTX-F and PnTX-E, similar to strains originating from the Pacific Ocean. This bloom was associated with unusual weather conditions such as frequent and prolonged droughts. Our findings indicate a close link between the V. rugosum bloom and a dermatitis outbreak among swimmers in Cienfuegos Bay. Phylogenetic evidence suggests a recent introduction of V. rugosum from the Pacific Ocean into Caribbean waters, possibly via ballast water. 
PY 2021
PD FEB
SO Science Of The Total Environment
SN 0048-9697
PU Elsevier BV
VL 757
UT 000604432900060
DI 10.1016/j.scitotenv.2020.143782
ID 77142
ER

EF