FN Archimer Export Format PT J TI Single-cell genomics of multiple uncultured stramenopiles reveals underestimated functional diversity across oceans BT AF SEELEUTHNER, Yoann MONDY, Samuel LOMBARD, Vincent CARRADEC, Quentin PELLETIER, Eric WESSNER, Marc LECONTE, Jade MANGOT, Jean-Francois POULAIN, Julie LABADIE, Karine LOGARES, Ramiro SUNAGAWA, Shinichi DE BERARDINIS, Veronique SALANOUBAT, Marcel DIMIER, Celine KANDELS-LEWIS, Stefanie PICHERAL, Marc SEARSON, Sarah PESANT, Stephane POULTON, Nicole STEPANAUSKAS, Ramunas BORK, Peer BOWLER, Chris HINGAMP, Pascal SULLIVAN, Matthew B. IUDICONE, Daniele MASSANA, Ramon AURY, Jean-Marc HENRISSAT, Bernard KARSENTI, Eric JAILLON, Olivier SIERACKI, Mike DE VARGAS, Colomban WINCKER, Patrick AS 1:1,2,3;2:1,2,3;3:4,5,6;4:1,2,3;5:1,2,3;6:1,2,3;7:1,2,3;8:7;9:1,2;10:1,2;11:7;12:8,9;13:2,3;14:2,3;15:10,11,12;16:8,13;17:14;18:15;19:16,17;20:18;21:18;22:8;23:;24:19;25:20,21;26:22;27:7;28:1;29:4,5,6,23;30:12;31:1,2,3;32:24;33:10,11;34:1,2,3;35:; FF 1:;2:;3:;4:;5:;6:;7:;8:;9:;10:;11:;12:;13:;14:;15:;16:;17:;18:;19:;20:;21:;22:;23:;24:;25:;26:;27:;28:;29:;30:;31:;32:;33:;34:;35:; C1 GENOSCOPE, CEA Inst Biol Francois Jacob, 2 Rue Gaston Cremieux, F-91057 Evry, France. CNRS, UMR 8030, CP5706, Evry, France. Univ Evry, UMR 8030, CP5706, Evry, France. CNRS, UMR 7257, F-13288 Marseille, France. Aix Marseille Univ, UMR 7257, F-13288 Marseille, France. INRA, USC 1408 AFMB, F-13288 Marseille, France. CSIC, Inst Ciencies Mar, Dept Marine Biol & Oceanog, E-08003 Barcelona, Catalonia, Spain. European Mol Biol Lab, Struct & Computat Biol, Meyerhofstr 1, D-69117 Heidelberg, Germany. Swiss Fed Inst Technol, Inst Microbiol, Dept Biol, Vladimir Prelog Weg 4, CH-8093 Zurich, Switzerland. CNRS, UMR 7144, Stn Biol Roscoff, Pl Georges Teissier, F-29680 Roscoff, France. Sorbonne Univ, UPMC, Univ Paris 06, UMR 7144,Stn Biol Roscoff, Pl Georges Teissier, F-29680 Roscoff, France. PSL Res Univ, Ecole Normale Super, IBENS, CNRS,INSERM,UMR 8197, U1024,46 Rue Ulm, F-75005 Paris, France. Directors Res European Mol Biol Lab, Meyerhofstr 1, D-69117 Heidelberg, Germany. Sorbonne Univ, UPMC, Univ Paris 06, CNRS,LOV,Observ Oceanol, F-06230 Villefranche Sur Mer, France. Univ Hawaii, Dept Oceanog, Honolulu, HI 96815 USA. Univ Bremen, PANGAEA Data Publisher Earth & Environm Sci, D-28359 Bremen, Germany. Univ Bremen, MARUM Ctr Marine Environm Sci, D-28359 Bremen, Germany. Bigelow Lab Ocean Sci, East Boothbay, ME 04544 USA. Aix Marseille Univ, Univ Toulon, CNRS, IRD,MIO UM 110, F-13288 Marseille, France. Ohio State Univ, Dept Microbiol, Columbus, OH 43210 USA. Ohio State Univ, Dept Civil Environm & Geodet Engn, Columbus, OH 43210 USA. Stn Zool Anton Dohrn, Villa Comunale, I-80121 Naples, Italy. King Abdulaziz Univ, Dept Biol Sci, Jeddah 21589, Saudi Arabia. Natl Sci Fdn, Arlington, VA 22230 USA. C2 GENOSCOPE, FRANCE CNRS, FRANCE UNIV EVRY, FRANCE CNRS, FRANCE UNIV AIX MARSEILLE, FRANCE INRA, FRANCE CSIC, SPAIN EMBL, GERMANY ETH ZURICH, SWITZERLAND CNRS, FRANCE UNIV PARIS 06, FRANCE ENS, FRANCE EMBL, GERMANY UNIV PARIS 06, FRANCE UNIV HAWAII, USA UNIV BREMEN, GERMANY UNIV BREMEN MARUM, GERMANY BIGELOW LAB OCEAN SCI, USA UNIV AIX MARSEILLE, FRANCE UNIV OHIO STATE, USA UNIV OHIO STATE, USA STN ZOOL ANTON DOHRN, ITALY KAUST, SAUDI ARABIA NATL SCI FDN, USA IN DOAJ IF 11.878 TC 60 UR https://archimer.ifremer.fr/doc/00660/77234/79044.pdf https://archimer.ifremer.fr/doc/00660/77234/79045.pdf https://archimer.ifremer.fr/doc/00660/77234/79046.pdf https://archimer.ifremer.fr/doc/00660/77234/79047.pdf https://archimer.ifremer.fr/doc/00660/77234/79048.xlsx https://archimer.ifremer.fr/doc/00660/77234/79049.xlsx https://archimer.ifremer.fr/doc/00660/77234/79050.xlsx https://archimer.ifremer.fr/doc/00660/77234/79052.pdf LA English DT Article AB Single-celled eukaryotes (protists) are critical players in global biogeochemical cycling of nutrients and energy in the oceans. While their roles as primary producers and grazers are well appreciated, other aspects of their life histories remain obscure due to challenges in culturing and sequencing their natural diversity. Here, we exploit single-cell genomics and metagenomics data from the circumglobal Tara Oceans expedition to analyze the genome content and apparent oceanic distribution of seven prevalent lineages of uncultured heterotrophic stramenopiles. Based on the available data, each sequenced genome or genotype appears to have a specific oceanic distribution, principally correlated with water temperature and depth. The genome content provides hypotheses for specialization in terms of cell motility, food spectra, and trophic stages, including the potential impact on their lifestyles of horizontal gene transfer from prokaryotes. Our results support the idea that prominent heterotrophic marine protists perform diverse functions in ocean ecology. PY 2018 PD JAN SO Nature Communications SN 2041-1723 PU Nature Publishing Group VL 9 IS 310 UT 000422915700005 DI 10.1038/s41467-017-02235-3 ID 77234 ER EF