FN Archimer Export Format
PT J
TI Enhancing PUFA-rich polar lipids in Tisochrysis lutea using adaptive laboratory evolution (ALE) with oscillating thermal stress
BT 
AF Gachelin, Manon
   Boutoute, Marc
   Carrier, Gregory
   Talec, Amélie
   Pruvost, Eric
   Guihéneuf, Freddy
   Bernard, Olivier
   Sciandra, Antoine
AS 1:1;2:1;3:2;4:1;5:1;6:1,3;7:4;8:1;
FF 1:;2:;3:PDG-RBE-BRM-LPBA;4:;5:;6:;7:;8:;
C1 Laboratoire d’Océanographie de Villefranche (LOV, UMR 7093), Sorbonne Universités, CNRS, Station zoologique, 181 Chemin du Lazaret, 06230, Villefranche-sur-Mer, France
   Laboratoire Physiologie et Biotechnologie des Algues (PBA), IFREMER, Nantes, France
   SAS Inalve, 181 chemin du lazaret, 06230, Villefranche-sur-Mer, France
   Biocore, INRIA Sophia Antipolis Méditerranée, Valbonne, France
C2 UNIV SORBONNE, FRANCE
   IFREMER, FRANCE
   SAS INALVE, FRANCE
   INRIA, FRANCE
SI NANTES
SE PDG-RBE-BRM-LPBA
IN WOS Ifremer UPR
   copubli-france
   copubli-univ-france
IF 5.56
TC 16
UR https://archimer.ifremer.fr/doc/00661/77269/84878.pdf
LA English
DT Article
DE ;Tisochrysis lutea;Polar lipids;Adaptive laboratory evolution (ALE);Temperature;DHA
AB Adaptive laboratory evolution is a powerful tool for microorganism improvement likely to produce enhanced microalgae better tailored to their industrial uses. In this work, 12 wild-type strains of Tisochrysis lutea were co-cultivated under increasing thermal stress for 6 months. Indeed, temperature was oscillating daily between a high and a low temperature, with increasing amplitude along the experiment. The goal was to enhance the polyunsaturated fatty acid content of the polar lipids. Samples were taken throughout the evolution experiment and cultivated in standardized conditions to analyze the evolution of the lipid profile. Genomic analysis of the final population shows that two strains survived. The lipid content doubled, impacting all lipid classes. The fatty acid analyses show a decrease in SFAs correlated with an increase in monounsaturated fatty acids (MUFAs), while changes in polyunsaturated fatty acid (PUFAs) vary between both photobioreactors. Hence, the proportion of C18-MUFAs (18:1 n-9) and most C18-PUFAs (18:2 n-6, 18:3 n-3, and 18:4 n-3) increased, suggesting their potential role in adjusting membrane fluidity to temperature shifts. Of particular interest, DHA in polar lipids tripled in the final population while the growth rate was not affected 
PY 2021
PD JAN
SO Applied Microbiology And Biotechnology
SN 0175-7598
PU Springer Science and Business Media LLC
VL 105
IS 1
UT 000629181000001
BP 301
EP 312
DI 10.1007/s00253-020-11000-4
ID 77269
ER

EF