FN Archimer Export Format
PT J
TI Effects of pH and Nutrients (Nitrogen) on Growth and Toxin Profile of the Ciguatera-Causing Dinoflagellate Gambierdiscus polynesiensis (Dinophyceae)
BT 
AF Longo, Sébastien
   SIBAT, Manoella
   Taiana Darius, Hélène
   HESS, Philipp
   Chinain, Mireille
AS 1:1;2:2;3:1;4:2;5:1;
FF 1:;2:PDG-ODE-DYNECO-PHYC;3:;4:PDG-ODE-DYNECO-PHYC;5:;
C1 Laboratory of Marine Biotoxins, Institut Louis Malardé-UMR241 EIO (IFREMER, ILM, IRD, UPF), 98713 Papeete, Tahiti, French Polynesia
   Phycotoxins Laboratory, IFREMER, Rue de I’lle d’Yeu, 44311 Nantes
C2 INST LOUIS MALARDE, FRANCE
   IFREMER, FRANCE
SI NANTES
SE PDG-ODE-DYNECO-PHYC
UM EIO
IN WOS Ifremer UPR
   WOS Cotutelle UMR
   DOAJ
   copubli-france
IF 2.13
TC 15
UR https://archimer.ifremer.fr/doc/00662/77382/78981.pdf
   https://archimer.ifremer.fr/doc/00662/77382/78982.pdf
LA English
DT Article
DE ;Gambierdiscus polynesiensis;French Polynesia;ciguatera;ciguatoxins;LC-MS;MS;toxin profile;nitrate;urea;culture medium acidification
AB Ciguatera poisoning is a foodborne disease caused by the consumption of seafood contaminated with ciguatoxins (CTXs) produced by dinoflagellates in the genera Gambierdiscus and Fukuyoa. Ciguatera outbreaks are expected to increase worldwide with global change, in particular as a function of its main drivers, including changes in sea surface temperature, acidification, and coastal eutrophication. In French Polynesia, G. polynesiensis is regarded as the dominant source of CTXs entering the food web. The effects of pH (8.4, 8.2, and 7.9), Nitrogen:Phosphorus ratios (24N:1P vs. 48N:1P), and nitrogen source (nitrates vs. urea) on growth rate, biomass, CTX levels, and profiles were examined in four clones of G. polynesiensis at different culture age (D10, D21, and D30). Results highlight a decrease in growth rate and cellular biomass at low pH when urea is used as a N source. No significant effect of pH, N:P ratio, and N source on the overall CTX content was observed. Up to ten distinct analogs of Pacific ciguatoxins (P-CTXs) could be detected by liquid chromatography-tandem mass spectrometry (LC-MS/MS) in clone NHA4 grown in urea, at D21. Amounts of more oxidized P-CTX analogs also increased under the lowest pH condition. These data provide interesting leads for the custom production of CTX standards. 
PY 2020
PD DEC
SO Toxins
SN 2072-6651
PU MDPI
VL 12
IS 12
UT 000602428800001
DI 10.3390/toxins12120767
ID 77382
ER

EF