FN Archimer Export Format
PT J
TI Heterotrophic Foraminifera Capable of Inorganic Nitrogen Assimilation
BT 
AF Bird, Clare
   LeKieffre, Charlotte
   Jauffrais, Thierry
   Meibom, Anders
   Geslin, Emmanuelle
   Filipsson, Helena L.
   Maire, Olivier
   Russell, Ann D.
   Fehrenbacher, Jennifer S.
AS 1:1,2;2:3,4;3:5;4:3,6;5:4;6:7;7:8,9;8:10;9:11;
FF 1:;2:;3:PDG-RBE-LEADNC;4:;5:;6:;7:;8:;9:;
C1 Biological and Environmental Sciences, Faculty of Natural Sciences, University of Stirling, Stirling, United Kingdom
   School of GeoSciences, Grant Institute, University of Edinburgh, Edinburgh, United Kingdom
   Laboratory for Biological Geochemistry, School of Architecture, Civil and Environmental Engineering (ENAC), Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland
   UMR CNRS 6112 LPG, Bio-Indicateurs Actuels et Fossiles, Université d’Angers, Angers, France
   Ifremer, IRD, Univ Nouvelle–Calédonie, Univ La Réunion, CNRS, UMR 9220 ENTROPIE, Nouméa, New Caledonia
   Centre for Advanced Surface Analysis, Institute of Earth Sciences, University of Lausanne, Lausanne, Switzerland
   Department of Geology, Lund University, Lund, Sweden
   Université de Bordeaux, EPOC, UMR 5805, Talence, France
   CNRS, EPOC, UMR 5805, Talence, France
   Department of Earth and Planetary Sciences, University of California, Davis, Davis, CA, United States
   College of Earth, Ocean, and Atmospheric Sciences, Oregon State University, Corvallis, OR, United States
C2 UNIV STIRLING, UK
   UNIV EDINBURGH, UK
   ECOLE POLYTECH FED LAUSANNE, SWITZERLAND
   UNIV ANGERS, FRANCE
   IFREMER, FRANCE
   UNIV LAUSANNE, SWITZERLAND
   UNIV LUND, SWEDEN
   UNIV BORDEAUX, FRANCE
   CNRS, FRANCE
   UNIV CALIF DAVIS, USA
   UNIV OREGON STATE, USA
SI NOUMEA
SE PDG-RBE-LEADNC
UM ENTROPIE
IN WOS Ifremer UMR
   DOAJ
   copubli-france
   copubli-europe
   copubli-univ-france
   copubli-int-hors-europe
IF 3.941
TC 5
UR https://archimer.ifremer.fr/doc/00662/77421/79087.pdf
   https://archimer.ifremer.fr/doc/00662/77421/79088.docx
   https://archimer.ifremer.fr/doc/00662/77421/79089.xlsx
   https://archimer.ifremer.fr/doc/00662/77421/79090.tif
   https://archimer.ifremer.fr/doc/00662/77421/79091.tif
LA English
DT Article
DE ;nitrogen cycle;heterotrophic protists;foraminifera;ammonium assimilation;heterotrophy;marine
AB Nitrogen availability often limits biological productivity in marine systems, where inorganic nitrogen, such as ammonium is assimilated into the food web by bacteria and photoautotrophic eukaryotes. Recently, ammonium assimilation was observed in kleptoplast-containing protists of the phylum foraminifera, possibly via the glutamine synthetase/glutamate synthase (GS/GOGAT) assimilation pathway imported with the kleptoplasts. However, it is not known if the ubiquitous and diverse heterotrophic protists have an innate ability for ammonium assimilation. Using stable isotope incubations (15N-ammonium and 13C-bicarbonate) and combining transmission electron microscopy (TEM) with quantitative nanoscale secondary ion mass spectrometry (NanoSIMS) imaging, we investigated the uptake and assimilation of dissolved inorganic ammonium by two heterotrophic foraminifera; a non-kleptoplastic benthic species, Ammonia sp., and a planktonic species, Globigerina bulloides. These species are heterotrophic and not capable of photosynthesis. Accordingly, they did not assimilate 13C-bicarbonate. However, both species assimilated dissolved 15N-ammonium and incorporated it into organelles of direct importance for ontogenetic growth and development of the cell. These observations demonstrate that at least some heterotrophic protists have an innate cellular mechanism for inorganic ammonium assimilation, highlighting a newly discovered pathway for dissolved inorganic nitrogen (DIN) assimilation within the marine microbial loop. 
PY 2020
PD DEC
SO Frontiers In Microbiology
SN 1664-302X
PU Frontiers Media SA
VL 11
UT 000599300200001
DI 10.3389/fmicb.2020.604979
ID 77421
ER

EF