FN Archimer Export Format
PT J
TI A new protocol using acidification for preserving DMSP in macroalgae and comparison with existing protocols
BT 
AF Bucciarelli, Eva
   STIGER-POUVREAU, Valerie
   Connan, Solène
AS 1:1;2:1;3:1;
FF 1:;2:;3:;
C1 Univ Brest CNRS IRD, Ifremer, LEMAR F‐29280 Plouzane, France
C2 UBO, FRANCE
UM LEMAR
IN WOS Cotutelle UMR
IF 3.173
TC 2
UR https://archimer.ifremer.fr/doc/00663/77503/79241.pdf
LA English
DT Article
DE ;acidification;DMSP;drying;freezing;macroalgae;storage protocol
AB Dimethylsulfoniopropionate (DMSP) plays many important physiological and ecological roles in macroalgae. The most common method to measure DMSP is by gas chromatography analysis of the dimethylsulfide (DMS) produced after NaOH hydrolysis (pH > 12). Storage of DMS, however, is not recommended for more than a week. We investigated if acidification can be a suitable method to preserve DMSP in macroalgal samples over three months of storage, compared to widely used protocols such as drying and freezing at ‐20°C. The DMSP content of green (Ulva sp. and Ulva compressa), red (Chondrus crispus) and brown (Bifurcaria bifurcata) macroalgae were analyzed 24 h after NaOH addition (control values); and after acidification (0.2 mol · L HCl‐1) for 24 h of fresh material, followed by NaOH addition for 24 h. These values were compared to measurements after 3‐month storage of samples that had been either dried in a heater (60°C for a night, and storage at room temperature), or frozen at ‐20°C, or kept in 0.2 mol · L HCl‐1. There was no significant difference between DMSP measurements on freshly collected material and after acidification of the samples, whether 24 h later or after 3 months of storage. This was in contrast with 3‐month storage protocols involving overnight drying at 60°C (75‐98% DMSP loss), and to a lesser degree freezing at ‐20°C (37‐80% DMSP loss). We thus advise to acidify macroalgal samples for preservation over long periods of time rather than drying or freezing, when assaying DMSP content. 
PY 2021
PD APR
SO Journal Of Phycology
SN 0022-3646
PU Wiley
VL 57
IS 2
UT 000614643900001
BP 689
EP 693
DI 10.1111/jpy.13113
ID 77503
ER

EF