Morphology, ultrastructure and molecular phylogeny of Johsia chumphonensis gen. et sp. nov. and Parvodinium parvulum comb. nov. (Peridiniopsidaceae, Dinophyceae)

Type Article
Date 2021-07
Language English
Author(s) Luo Zhaohe1, Mertens KennethORCID2, Gu Haifeng1, 3, Wang Na1, Wu Yiran1, Uttayarnmanee Praderm4, Pransilpa Mitila5, Roeroe Kakaskasen Andreas6
Affiliation(s) 1 : Third Institute of Oceanography, Ministry of Natural Resources , Xiamen 361005, China
2 : Ifremer, LER BO, Station de Biologie Marine, Place de la Croix , BP40537, F-29185 Concarneau CEDEX, France
3 : School of Marine Sciences, Nanjing University of Information Science and Technology , Nanjing 210044, China
4 : Marine and Coastal Resources Research and Development Center , Central Gulf of Thailand, Department of Marine and Coastal Resources, Chumphon, 86000, Thailand
5 : Marine and Coastal Resources Research and Development Center , Eastern Gulf of Thailand, Department of Marine and Coastal Resources, Rayong, 21170, Thailand
6 : Sam Ratulangi University , Sulawesi Utara, Manado, 95115, Indonesia
Source European Journal Of Phycology (0967-0262) (Informa UK Limited), 2021-07 , Vol. 56 , N. 3 , P. 324-336
DOI 10.1080/09670262.2020.1829078
WOS© Times Cited 2
Keyword(s) Cyst, dinoflagellate, eyespot, freshwater, immotile cell, Peridinium parvulum
Abstract

The family Peridiniopsidaceae encompasses mainly freshwater species of the genera Peridiniopsis, Palatinus and Parvodinium. Only one benthic, marine species ‘Scrippsiella’ hexapraecingula has been attributed to this family. Here we established five strains by isolating single Parvodinium-like cells from the marine Gulf of Thailand, Hainan Island waters (China), off Manado (Indonesia) and from a freshwater reservoir in Fuzhou (China). All strains were examined with light, scanning and transmission electron microscopy, and their SSU, ITS-5.8S and partial LSU rRNA regions were sequenced. Four marine strains share a plate formula of Po, cp, X, 4′, 2a, 6′′, 6C, 4S, 5′′′, 2′′′′ and are herein attributed to a new genus Johsia as J. chumphonensis gen. et sp. nov. Its theca is characterized by an epitheca 1.5 times as long as the hypotheca in dorsal view and a 2a plate about half the size of 1a. A type B eyespot was observed in J. chumphonensis comprising two rows of lipid globules within a chloroplast with a single row of crystals overlying the eyespot. Production of spherical cysts was observed in culture. The freshwater strain shows a plate pattern of Po, cp, X, 4′, 2a, 7′′, 6C, 5S, 5′′′, 2′′′′, characterized by two unequal antapical plates and a lack of antapical spines, fitting the description of Peridinium parvulum, which was transferred to Parvodinium as P. parvulum comb. nov. In this species, a type A eyespot was observed comprising four rows of lipid globules within a chloroplast. A molecular phylogeny was inferred based on concatenated data from SSU, ITS-5.8S and partial LSU rRNA gene sequences using maximum likelihood and Bayesian inference. Our results show that Johsia is nested within the Peridiniopsidaceae and is a sister clade to Peridiniopsis borgei and the strain UTEX1948 identified as ‘Scrippsiella’ hexapraecingula with three anterior intercalary plates.

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Luo Zhaohe, Mertens Kenneth, Gu Haifeng, Wang Na, Wu Yiran, Uttayarnmanee Praderm, Pransilpa Mitila, Roeroe Kakaskasen Andreas (2021). Morphology, ultrastructure and molecular phylogeny of Johsia chumphonensis gen. et sp. nov. and Parvodinium parvulum comb. nov. (Peridiniopsidaceae, Dinophyceae). European Journal Of Phycology, 56(3), 324-336. Publisher's official version : https://doi.org/10.1080/09670262.2020.1829078 , Open Access version : https://archimer.ifremer.fr/doc/00663/77516/