FN Archimer Export Format
PT J
TI Morphology, ultrastructure and molecular phylogeny of   Johsia chumphonensis   gen. et sp. nov. and   Parvodinium parvulum   comb. nov. (Peridiniopsidaceae, Dinophyceae)
BT 
AF Luo, Zhaohe
   Mertens, Kenneth
   Gu, Haifeng
   Wang, Na
   Wu, Yiran
   Uttayarnmanee, Praderm
   Pransilpa, Mitila
   Roeroe, Kakaskasen Andreas
AS 1:1;2:2;3:1,3;4:1;5:1;6:4;7:5;8:6;
FF 1:;2:PDG-ODE-LITTORAL-LERBO;3:;4:;5:;6:;7:;8:;
C1 Third Institute of Oceanography, Ministry of Natural Resources , Xiamen 361005, China
   Ifremer, LER BO, Station de Biologie Marine, Place de la Croix , BP40537, F-29185 Concarneau CEDEX, France
   School of Marine Sciences, Nanjing University of Information Science and Technology , Nanjing 210044, China
   Marine and Coastal Resources Research and Development Center , Central Gulf of Thailand, Department of Marine and Coastal Resources, Chumphon, 86000, Thailand
   Marine and Coastal Resources Research and Development Center , Eastern Gulf of Thailand, Department of Marine and Coastal Resources, Rayong, 21170, Thailand
   Sam Ratulangi University , Sulawesi Utara, Manado, 95115, Indonesia
C2 SOA, CHINA
   IFREMER, FRANCE
   UNIV NANJING, CHINA
   DMCR, THAILAND
   DMCR, THAILAND
   UNIV SAM RATULANGI, INDONESIA
SI CONCARNEAU
SE PDG-ODE-LITTORAL-LERBO
IN WOS Ifremer UPR
   copubli-int-hors-europe
   copubli-sud
IF 2.667
TC 3
UR https://archimer.ifremer.fr/doc/00663/77516/79599.pdf
LA English
DT Article
DE ;Cyst;dinoflagellate;eyespot;freshwater;immotile cell;Peridinium parvulum
AB The family Peridiniopsidaceae encompasses mainly freshwater species of the genera Peridiniopsis, Palatinus and Parvodinium. Only one benthic, marine species ‘Scrippsiella’ hexapraecingula has been attributed to this family. Here we established five strains by isolating single Parvodinium-like cells from the marine Gulf of Thailand, Hainan Island waters (China), off Manado (Indonesia) and from a freshwater reservoir in Fuzhou (China). All strains were examined with light, scanning and transmission electron microscopy, and their SSU, ITS-5.8S and partial LSU rRNA regions were sequenced. Four marine strains share a plate formula of Po, cp, X, 4′, 2a, 6′′, 6C, 4S, 5′′′, 2′′′′ and are herein attributed to a new genus Johsia as J. chumphonensis gen. et sp. nov. Its theca is characterized by an epitheca 1.5 times as long as the hypotheca in dorsal view and a 2a plate about half the size of 1a. A type B eyespot was observed in J. chumphonensis comprising two rows of lipid globules within a chloroplast with a single row of crystals overlying the eyespot. Production of spherical cysts was observed in culture. The freshwater strain shows a plate pattern of Po, cp, X, 4′, 2a, 7′′, 6C, 5S, 5′′′, 2′′′′, characterized by two unequal antapical plates and a lack of antapical spines, fitting the description of Peridinium parvulum, which was transferred to Parvodinium as P. parvulum comb. nov. In this species, a type A eyespot was observed comprising four rows of lipid globules within a chloroplast. A molecular phylogeny was inferred based on concatenated data from SSU, ITS-5.8S and partial LSU rRNA gene sequences using maximum likelihood and Bayesian inference. Our results show that Johsia is nested within the Peridiniopsidaceae and is a sister clade to Peridiniopsis borgei and the strain UTEX1948 identified as ‘Scrippsiella’ hexapraecingula with three anterior intercalary plates. 
PY 2021
PD JUN
SO European Journal Of Phycology
SN 0967-0262
PU Informa UK Limited
VL 56
IS 3
UT 000597381200001
BP 324
EP 336
DI 10.1080/09670262.2020.1829078
ID 77516
ER

EF