Seasonality of archaeal proteorhodopsin and associated Marine Group IIb ecotypes (Ca. Poseidoniales) in the North Western Mediterranean Sea

Type Article
Date 2021-05
Language English
Author(s) Pereira Olivier1, Hochart Corentin1, Boeuf Dominique2, Auguet Jean-Christophe3, Debroas Didier4, Galand Pierre E.1
Affiliation(s) 1 : Sorbonne Universités, CNRS, Laboratoire d’Ecogéochimie des Environnements Benthiques (LECOB), Observatoire Océanologique, Banyuls sur Mer, France
2 : Daniel K. Inouye Center for Microbial Oceanography, Research and Education, School of Ocean and Earth Science and Technology, University of Hawai‘i at Mānoa, Honolulu, HI, United States, Honolulu, HI, 96822, USA
3 : MARBEC, Université de Montpellier, CNRS, Ifremer, IRD, Montpellier, France, Montpellier, France
4 : Université Clermont Auvergne, CNRS, Laboratoire Microorganismes: Genome et Environnement, 63000, Clermont-Ferrand, France
Source Isme Journal (1751-7362) (Springer Science and Business Media LLC), 2021-05 , Vol. 15 , N. 5 , P. 1302-1316
DOI 10.1038/s41396-020-00851-4
WOS© Times Cited 1
Abstract

The Archaea Marine Group II (MGII) is widespread in the world’s ocean where it plays an important role in the carbon cycle. Despite recent discoveries on the group’s metabolisms, the ecology of this newly proposed order (Candidatus Poseidoniales) remains poorly understood. Here we used a combination of time-series metagenome-assembled genomes (MAGs) and high-frequency 16S rRNA data from the NW Mediterranean Sea to test if the taxonomic diversity within the MGIIb family (Candidatus Thalassarchaeaceae) reflects the presence of different ecotypes. The MAGs’ seasonality revealed a MGIIb family composed of different subclades that have distinct lifestyles and physiologies. The vitamin metabolisms were notably different between ecotypes with, in some, a possible link to sunlight’s energy. Diverse archaeal proteorhodopsin variants, with unusual signature in key amino acid residues, had distinct seasonal patterns corresponding to changing day length. In addition, we show that in summer, archaea, as opposed to bacteria, disappeared completely from surface waters. Our results shed light on the diversity and the distribution of the euryarchaeotal proteorhodopsin, and highlight that MGIIb is a diverse ecological group. The work shows that time-series based studies of the taxonomy, seasonality, and metabolisms of marine prokaryotes is critical to uncover their diverse role in the ocean.

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Pereira Olivier, Hochart Corentin, Boeuf Dominique, Auguet Jean-Christophe, Debroas Didier, Galand Pierre E. (2021). Seasonality of archaeal proteorhodopsin and associated Marine Group IIb ecotypes (Ca. Poseidoniales) in the North Western Mediterranean Sea. Isme Journal, 15(5), 1302-1316. Publisher's official version : https://doi.org/10.1038/s41396-020-00851-4 , Open Access version : https://archimer.ifremer.fr/doc/00663/77526/