FN Archimer Export Format
PT J
TI Imaging the uptake of nitrogen-fixing bacteria into larvae of the coral Acropora millepora
BT 
AF LEMA, Anais Kimberley
   CLODE, Peta L.
   KILBURN, Matt R.
   THORNTON, Ruth
   WILLIS, Bette L.
   BOURNE, David G.
AS 1:1,2,3,4,5;2:6,7;3:6;4:8;5:1,2,4;6:3,4;
FF 1:PDG-ODE-DYNECO-PELAGOS;2:;3:;4:;5:;6:;
C1 James Cook Univ, ARC Ctr Excellence Coral Reef Studies, Townsville, Qld 4811, Australia.
   James Cook Univ, Coll Marine & Environm Sci, Townsville, Qld 4811, Australia.
   Australian Inst Marine Sci, Ctr Marine Microbiol & Genet, PMB 3, Townsville, Qld 4810, Australia.
   James Cook Univ, AIMS JCU, Townsville, Qld 4811, Australia.
   IFREMER, Dyneco Pelagos, Plouzane, France.
   Univ Western Australia, Ctr Microscopy Characterisat & Anal, Crawley, WA, Australia.
   Univ Western Australia, Oceans Inst, Crawley, WA, Australia.
   Univ Western Australia, Sch Paediat & Child Heath, Crawley, WA, Australia.
C2 UNIV JAMES COOK, AUSTRALIA
   UNIV JAMES COOK, AUSTRALIA
   AUSTRALIAN INST MARINE SCI, AUSTRALIA
   UNIV JAMES COOK, AUSTRALIA
   IFREMER, FRANCE
   UNIV WESTERN AUSTRALIA, AUSTRALIA
   UNIV WESTERN AUSTRALIA, AUSTRALIA
   UNIV WESTERN AUSTRALIA, AUSTRALIA
SI BREST
SE PDG-ODE-DYNECO-PELAGOS
IN WOS Ifremer jusqu'en 2018
   copubli-int-hors-europe
IF 9.664
TC 31
UR https://archimer.ifremer.fr/doc/00664/77573/79543.pdf
   https://archimer.ifremer.fr/doc/00664/77573/79544.docx
   https://archimer.ifremer.fr/doc/00664/77573/79545.docx
   https://archimer.ifremer.fr/doc/00664/77573/79546.docx
   https://archimer.ifremer.fr/doc/00664/77573/79547.jpg
   https://archimer.ifremer.fr/doc/00664/77573/79548.jpg
   https://archimer.ifremer.fr/doc/00664/77573/79549.jpg
   https://archimer.ifremer.fr/doc/00664/77573/79550.jpg
   https://archimer.ifremer.fr/doc/00664/77573/79551.jpg
   https://archimer.ifremer.fr/doc/00664/77573/79552.pdf
LA English
DT Article
AB Diazotrophic bacteria are instrumental in generating biologically usable forms of nitrogen by converting abundant dinitrogen gas (N-2) into available forms, such as ammonium. Although nitrogen is crucial for coral growth, direct observation of associations between diazotrophs and corals has previously been elusive. We applied fluorescence in situ hybridization (FISH) and nanoscale secondary ion mass spectrometry to observe the uptake of N-15-enriched diazotrophic Vibrio sp. isolated from Acropora millepora into conspecific coral larvae. Incorporation of Vibrio sp. cells was observed in coral larvae after 4-h incubation with enriched bacteria. Uptake was restricted to the aboral epidermis of larvae, where Vibrio cells clustered in elongated aggregations. Other bacterial associates were also observed in epidermal areas in FISH analyses. Although the fate and role of these bacteria requires additional investigation, this study describes a powerful approach to further explore cell associations and nutritional pathways in the early life stages of the coral holobiont. 
PY 2016
PD JUN
SO Isme Journal
SN 1751-7362
PU Nature Publishing Group
VL 10
IS 7
UT 000378292100022
BP 1804
EP 1808
DI 10.1038/ismej.2015.229
ID 77573
ER

EF