FN Archimer Export Format
PT J
TI Differential Mortality and High Viral Load in Naive Pacific Oyster Families Exposed to OsHV-1 Suggests Tolerance Rather than Resistance to Infection
BT 
AF Agnew, M. Victoria
   Friedman, Carolyn S.
   Langdon, Christopher
   Divilov, Konstantin
   Schoolfield, Blaine
   Morga, Benjamin
   Degremont, Lionel
   Dhar, Arun K.
   Kirkland, Peter
   Dumbauld, Brett
   Burge, Colleen A.
AS 1:1;2:2;3:3;4:3;5:3;6:4;7:4;8:5;9:6;10:7;11:1;
FF 1:;2:;3:;4:;5:;6:PDG-RBE-SGMM-LGPMM;7:PDG-RBE-SGMM-LGPMM;8:;9:;10:;11:;
C1 Institute of Marine and Environmental Technology, University of Maryland Baltimore County, Baltimore, MD 21202, USA
   School of Aquatic and Fishery Sciences, University of Washington, Seattle, WA 98195, USA
   Department of Fisheries and Wildlife, Coastal Oregon Marine Experiment Station, Hatfield Marine Science Center, Newport, OR 97365, USA
   Ifremer, SG2M, LGPMM, 17390 La Tremblade, France
   Aquaculture Pathology Laboratory, School of Animal and Comparative Biomedical Sciences, University of Arizona, Tucson, AZ 85721, USA
   NSW Department of Primary Industries, Elizabeth Macarthur Agricultural Institute, Menangle, NSW 2568, Australia
   Hatfield Marine Science Center, USDA-ARS, Newport, OR 97365, USA
C2 UNIV MARYLAND, USA
   UNIV WASHINGTON, USA
   COMES, USA
   IFREMER, FRANCE
   UNIV ARIZONA, USA
   EMAI, AUSTRALIA
   USDA, USA
SI LA TREMBLADE
SE PDG-RBE-SGMM-LGPMM
IN WOS Ifremer UPR
   DOAJ
   copubli-int-hors-europe
IF 3.405
TC 9
UR https://archimer.ifremer.fr/doc/00665/77739/79862.pdf
   https://archimer.ifremer.fr/doc/00665/77739/79863.zip
LA English
DT Article
DE ;OsHV-1;mu vars;oyster;virus;tolerance;virulence;qPCR
AB Pacific oysters, Crassostrea gigas, are one of the most productive aquaculture species in the world. However, they are threatened by the spread of Ostreid herpesvirus-1 (OsHV-1) and its microvariants (collectively “µvars”), which cause mass mortalities in all life stages of Pacific oysters globally. Breeding programs have been successful in reducing mortality due to OsHV-1 variants following viral outbreaks; however, an OsHV-1-resistant oyster line does not yet exist in the United States (US), and it is unknown how OsHV-1 µvars will affect US oyster populations compared to the current variant, which is similar to the OsHV-1 reference, found in Tomales Bay, CA. The goals of this study were to investigate the resistance of C. gigas juveniles produced by the Molluscan Broodstock Program (MBP) to three variants of OsHV-1: a California reference OsHV-1, an Australian µvar, and a French µvar. This is the first study to directly compare OsHV-1 µvars to a non-µvar. The survival probability of oysters exposed to the French (FRA) or Australian (AUS) µvar was significantly lower (43% and 71%, respectively) than to the reference variant and controls (96%). No oyster family demonstrated resistance to all three OsHV-1 variants, and many surviving oysters contained high copy numbers of viral DNA (mean ~3.53 × 108). These results indicate that the introduction of OsHV-1 µvars could have substantial effects on US Pacific oyster aquaculture if truly resistant lines are not achieved, and highlight the need to consider resistance to infection in addition to survival as traits in breeding programs to reduce the risk of the spread of OsHV-1 variants 
PY 2020
PD DEC
SO Pathogens
SN 2076-0817
PU MDPI AG
VL 9
IS 12
UT 000602302400001
DI 10.3390/pathogens9121057
ID 77739
ER

EF