Molecular Characterization of Novel Cryptosporidium Fish Genotypes in Edible Marine Fish
Type | Article | ||||||||
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Date | 2020-12 | ||||||||
Language | English | ||||||||
Author(s) | Certad Gabriela![]() ![]() ![]() |
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Affiliation(s) | 1 : Institut Pasteur de Lille, U1019–UMR 9017–CIIL–Centre d’Infection et d’Immunité de Lille, Université de Lille, CNRS, Inserm, CHU Lille, F-59000 Lille, France 2 : Délégation à la Recherche Clinique et à l’Innovation, Groupement des Hôpitaux de l’Institut Catholique de Lille, F-59462 Lomme, France 3 : Harry Butler Institute, Murdoch University, Perth 6150, Australia 4 : Service d’Anatomie et de Cytologie Pathologiques, Groupement des Hôpitaux de l’Institut Catholique de Lille (GHICL), F-59000 Lille, France 5 : Laboratoire Ecologie et Biodiversité, Institut Catholique de Lille, Faculté de Gestion Economie et Sciences, F-59000 Lille, France |
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Source | Microorganisms (2076-2607) (MDPI AG), 2020-12 , Vol. 8 , N. 12 , P. 2014 (9p.) | ||||||||
DOI | 10.3390/microorganisms8122014 | ||||||||
WOS© Times Cited | 8 | ||||||||
Note | This article belongs to the Special Issue Epidemiology, Transmission, Cell Biology and Pathogenicity of Cryptosporidium | ||||||||
Keyword(s) | piscine Cryptosporidium, edible marine fish, genetic characterization, 18S rDNA gene, actin gene, molecular phylogeny | ||||||||
Abstract | Current knowledge of Cryptosporidium species/genotypes in marine fish is limited. Following phylogenetic analysis at the 18S rDNA locus, a recent study identified six new genotypes of Cryptosporidium colonizing edible fish found in European seas. Of these, five grouped in a clade together (#Cryptofish 1–5) and one grouped separately (#Cryptofish 7). In the present study, after phylogenetic analyses of #Cryptofish1, #Cryptofish2, #Cryptofish4, #Cryptofish5 and #Cryptofish7 at the actin locus, the presence of two major clades was confirmed. In addition, when possible, longer 18S amplicons were generated. In conclusion, the small genetic distances between these genotypes designated as a novel marine genotype I (#Cryptofish 1-5) suggest that they may be genetic variants of the same species, while the designated novel marine genotype 2 (#Cryptofish 7) is clearly representative of a separate species. |
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