TY - JOUR T1 - Molecular Characterization of Novel Cryptosporidium Fish Genotypes in Edible Marine Fish A1 - Certad,Gabriela A1 - Zahedi,Alireza A1 - Gantois,Nausicaa A1 - Sawant,Manasi A1 - Creusy,Colette A1 - Duval,Erika A1 - Benamrouz-Vanneste,Sadia A1 - Ryan,Una A1 - Viscogliosi,Eric AD - Institut Pasteur de Lille, U1019–UMR 9017–CIIL–Centre d’Infection et d’Immunité de Lille, Université de Lille, CNRS, Inserm, CHU Lille, F-59000 Lille, France AD - Délégation à la Recherche Clinique et à l’Innovation, Groupement des Hôpitaux de l’Institut Catholique de Lille, F-59462 Lomme, France AD - Harry Butler Institute, Murdoch University, Perth 6150, Australia AD - Service d’Anatomie et de Cytologie Pathologiques, Groupement des Hôpitaux de l’Institut Catholique de Lille (GHICL), F-59000 Lille, France AD - Laboratoire Ecologie et Biodiversité, Institut Catholique de Lille, Faculté de Gestion Economie et Sciences, F-59000 Lille, France UR - https://archimer.ifremer.fr/doc/00665/77741/ DO - 10.3390/microorganisms8122014 KW - piscine Cryptosporidium KW - edible marine fish KW - genetic characterization KW - 18S rDNA gene KW - actin gene KW - molecular phylogeny N2 - Current knowledge of Cryptosporidium species/genotypes in marine fish is limited. Following phylogenetic analysis at the 18S rDNA locus, a recent study identified six new genotypes of Cryptosporidium colonizing edible fish found in European seas. Of these, five grouped in a clade together (#Cryptofish 1–5) and one grouped separately (#Cryptofish 7). In the present study, after phylogenetic analyses of #Cryptofish1, #Cryptofish2, #Cryptofish4, #Cryptofish5 and #Cryptofish7 at the actin locus, the presence of two major clades was confirmed. In addition, when possible, longer 18S amplicons were generated. In conclusion, the small genetic distances between these genotypes designated as a novel marine genotype I (#Cryptofish 1-5) suggest that they may be genetic variants of the same species, while the designated novel marine genotype 2 (#Cryptofish 7) is clearly representative of a separate species. Y1 - 2020/12 PB - MDPI AG JF - Microorganisms SN - 2076-2607 VL - 8 IS - 12 ID - 77741 ER -