FN Archimer Export Format PT J TI Molecular Characterization of Novel Cryptosporidium Fish Genotypes in Edible Marine Fish BT AF Certad, Gabriela Zahedi, Alireza Gantois, Nausicaa Sawant, Manasi Creusy, Colette Duval, Erika Benamrouz-Vanneste, Sadia Ryan, Una Viscogliosi, Eric AS 1:1,2;2:3;3:1;4:1;5:4;6:4;7:1,5;8:3;9:1; FF 1:;2:;3:;4:;5:;6:;7:;8:;9:; C1 Institut Pasteur de Lille, U1019–UMR 9017–CIIL–Centre d’Infection et d’Immunité de Lille, Université de Lille, CNRS, Inserm, CHU Lille, F-59000 Lille, France Délégation à la Recherche Clinique et à l’Innovation, Groupement des Hôpitaux de l’Institut Catholique de Lille, F-59462 Lomme, France Harry Butler Institute, Murdoch University, Perth 6150, Australia Service d’Anatomie et de Cytologie Pathologiques, Groupement des Hôpitaux de l’Institut Catholique de Lille (GHICL), F-59000 Lille, France Laboratoire Ecologie et Biodiversité, Institut Catholique de Lille, Faculté de Gestion Economie et Sciences, F-59000 Lille, France C2 INST PASTEUR, FRANCE GHICL, FRANCE UNIV MURDOCH, AUSTRALIA GHICL, FRANCE INST CATHOL LILLE, FRANCE IN DOAJ IF 4.167 TC 9 UR https://archimer.ifremer.fr/doc/00665/77741/79864.pdf LA English DT Article CR EVHOE EVALUATION HALIEUTIQUE OUEST DE L'EUROPE INTERNATIONAL BOTTOM TRAWL SURVEY (IBTS) PELGAS PELMED - PELAGIQUES MEDITERRANÉE DE ;piscine Cryptosporidium;edible marine fish;genetic characterization;18S rDNA gene;actin gene;molecular phylogeny AB Current knowledge of Cryptosporidium species/genotypes in marine fish is limited. Following phylogenetic analysis at the 18S rDNA locus, a recent study identified six new genotypes of Cryptosporidium colonizing edible fish found in European seas. Of these, five grouped in a clade together (#Cryptofish 1–5) and one grouped separately (#Cryptofish 7). In the present study, after phylogenetic analyses of #Cryptofish1, #Cryptofish2, #Cryptofish4, #Cryptofish5 and #Cryptofish7 at the actin locus, the presence of two major clades was confirmed. In addition, when possible, longer 18S amplicons were generated. In conclusion, the small genetic distances between these genotypes designated as a novel marine genotype I (#Cryptofish 1-5) suggest that they may be genetic variants of the same species, while the designated novel marine genotype 2 (#Cryptofish 7) is clearly representative of a separate species. PY 2020 PD DEC SO Microorganisms SN 2076-2607 PU MDPI AG VL 8 IS 12 UT 000602304200001 DI 10.3390/microorganisms8122014 ID 77741 ER EF