FN Archimer Export Format
PT J
TI Mechanisms of acclimation to hypersalinity in two European sea bass lineages: a focus on the kidney function
BT 
AF Cao, Quanquan
   Giffard-Mena, Ivone
   Blondeau-Bidet, Eva
   Hermet, Sophie
   Hu, Yau-Chung
   Lee, Tsung-Han
   Lorin-Nebel, Catherine
AS 1:1;2:2;3:1;4:1;5:3;6:3;7:1;
FF 1:;2:;3:;4:;5:;6:;7:;
C1 Univ Montpellier, MARBEC (CNRS, IFREMER, IRD, UM), 34095 Montpellier, France
   Universidad Autónoma de Baja California, Facultad de Ciencias Marinas, 22860 Ensenada, Baja California, Mexico
   Department of Life Sciences, National Chung Hsing University, Taichung 402, Taiwan
C2 UNIV MONTPELLIER, FRANCE
   UNIV AUTONOMA BAJA CALIFORNIA, MEXICO
   UNIV NATL CHUNG HSING, TAIWAN
UM MARBEC
IN WOS Cotutelle UMR
   copubli-int-hors-europe
   copubli-sud
IF 5.135
TC 2
UR https://archimer.ifremer.fr/doc/00666/77766/93564.pdf
LA English
DT Article
DE ;Hypersalinity;Kidney;Dicentrarchus labrax;Intraspecific comparison;Osmoregulation
AB European sea bass (Dicentrarchus labrax), a major aquaculture species, is distributed along the coasts of the North-Eastern Atlantic Ocean, Mediterranean and Black Sea. D. labrax enter lagoons and estuaries where salinity fluctuates and sometimes reaches levels over 60‰, notably in Mediterranean lagoons. Keeping in mind that European sea bass are genetically subdivided in an Atlantic and a Mediterranean lineage, we compared fish from Atlantic (A) and West Mediterranean (M) populations regarding their capacity to tolerate hypersalinity with a focus on the kidney, a key organ involved in water reabsorption at high salinity. Fish were analyzed following a two-week transfer from seawater (SW, 36‰) to either seawater (SW, 36‰) or hypersaline water (HW, 55‰). Plasma osmolality was significantly increased in the MHW group compared to the other groups. Plasma sodium levels were significantly increased in hypersaline water compared to seawater in both lineages whereas plasma chloride levels showed an opposite trend. In order to estimate water filtration at the kidney level, the size of renal glomeruli was investigated and showed a decreased glomerulus perimeter and area in hypersaline water compared to seawater. NKA was highly expressed in all kidney tubules notably collecting tubules and ducts. There was an effect of salinity on renal nka α1a mRNA expression with slightly lower transcript levels at 55‰ compared to 36‰. Relative protein amounts and activity of NKA however were significantly higher in fish exposed to hypersalinity regardless of their origin. AQP1a immunolabeling differed between proximal tubules subtypes and only faint AQP1a was detected in subapical parts of cells lining collecting ducts. The transcript levels of renal aqp 1a were lower in the HW group than the SW group whereas the expression of other aqp paralogs (aqp 1b, aqp 8b) did not change according to the analyzed conditions. This study showed an efficient acclimation of sea bass to high salinity by increasing active ion transport at the kidney and by decreasing the size of filtering glomeruli to minimize water loss through urine. Despite Mediterranean D. labrax are supposed to more often encounter high salinities in their habitat, their high blood osmolality in hypersaline water indicates that their overall response to hypersalinity seems not improved compared to the Atlantic lineage. However, at the kidney level, the traits analyzed differ slightly between genetic lineages, potentially as a response to high blood osmolalities in MHW. 
PY 2021
PD MAR
SO Aquaculture
SN 0044-8486
PU Elsevier BV
VL 534
UT 000614765100005
DI 10.1016/j.aquaculture.2020.736305
ID 77766
ER

EF