FN Archimer Export Format
PT J
TI "Frozen evolution" of an RNA virus suggests accidental release as a potential cause of arbovirus re-emergence
BT 
AF PASCALL, David J.
   NOMIKOU, Kyriaki
   BREARD, Emmanuel
   ZIENTARA, Stephan
   FILIPE, Ana da Silva
   HOFFMANN, Bernd
   JACQUOT, Maude
   SINGER, Joshua B.
   DE CLERCQ, Kris
   BOTNER, Anette
   SAILLEAU, Corinne
   VIAROUGE, Cyril
   BATTEN, Carrie
   PUGGIONI, Giantonella
   LIGIOS, Ciriaco
   SAVINI, Giovanni
   VAN RIJN, Piet A.
   MERTENS, Peter P. C.
   BIEK, Roman
   PALMARINI, Massimo
AS 1:1;2:2,3;3:4;4:4;5:2;6:5;7:6,7;8:2;9:8;10:9,10;11:4;12:4;13:11;14:12;15:12;16:13;17:14,15;18:3,11;19:1;20:2;
FF 1:;2:;3:;4:;5:;6:;7:;8:;9:;10:;11:;12:;13:;14:;15:;16:;17:;18:;19:;20:;
C1 Univ Glasgow, Boyd Orr Ctr Populat & Ecosyst Hlth, Inst Biodivers Anim Hlth & Comparat Med, Glasgow, Lanark, Scotland.
   Univ Glasgow, MRC, Ctr Virus Res, Glasgow, Lanark, Scotland.
   Univ Nottingham, Sch Vet Med & Sci, Loughborough, Leics, England.
   Univ Paris Est, ANSES, Lab Sante Anim Alfort, UMR Virol,INRA,Ecole Natl Vet Alfort, Maisons Alfort, France.
   Friedrich Loeffler Inst, Inst Diagnost Virol, Greifswald, Germany.
   Univ Brussels, Spatial Epidemiol Lab SpELL, Brussels, Belgium.
   INRAE VetAgro Sup, UMR Epidemiol Anim & Zoonot Dis, St Genes Champanelle, France.
   Sciensano, Infect Dis Anim Exot & Particular Dis, Brussels, Belgium.
   Univ Copenhagen, Dept Vet & Anim Sci, Sect Vet Clin Microbiol, Copenhagen, Denmark.
   Statens Serum Inst, Dept Virus & Microbiol Special Diagnost, Copenhagen, Denmark.
   Pirbright Inst, Woking, Surrey, England.
   Ist Zooprofilatt Sperimentale Sardegna, Via Duca Abruzzi, Sassari, Italy.
   Ist Zooprofilatt Sperimentale Abruzzo & Molise IZ, Teramo, Italy.
   Wageningen Biovet Res WBVR, Dept Virol, Lelystad, Netherlands.
   North West Univ, Ctr Human Metabol, Dept Biochem, Potchefstroom, South Africa.
C2 UNIV GLASGOW, UK
   UNIV GLASGOW, UK
   UNIV NOTTINGHAM, UK
   UNIV PARIS EST, FRANCE
   FRIEDRICH LOEFFLER INST, GERMANY
   UNIV BRUSSELS, BELGIUM
   INRAE, FRANCE
   SCIENSANO, BELGIUM
   UNIV COPENHAGEN, DENMARK
   STATENS SERUM INST, DENMARK
   PIRBRIGHT INST, UK
   IZSSAR, ITALY
   IZSSAM, ITALY
   UNIV WAGENINGEN, NETHERLANDS
   UNIV NORTH WEST, SOUTH AFRICA
IN DOAJ
IF 8.029
TC 11
UR https://archimer.ifremer.fr/doc/00667/77881/80035.pdf
   https://archimer.ifremer.fr/doc/00667/77881/80036.cpgz
   https://archimer.ifremer.fr/doc/00667/77881/80037.cpgz
   https://archimer.ifremer.fr/doc/00667/77881/80038.cpgz
   https://archimer.ifremer.fr/doc/00667/77881/80039.tif
   https://archimer.ifremer.fr/doc/00667/77881/80040.tif
   https://archimer.ifremer.fr/doc/00667/77881/80041.xlsx
   https://archimer.ifremer.fr/doc/00667/77881/80064.pdf
LA English
DT Article
AB The mechanisms underlying virus emergence are rarely well understood, making the appearance of outbreaks largely unpredictable. Bluetongue virus serotype 8 (BTV-8), an arthropod-borne virus of ruminants, emerged in livestock in northern Europe in 2006, spreading to most European countries by 2009 and causing losses of billions of euros. Although the outbreak was successfully controlled through vaccination by early 2010, puzzlingly, a closely related BTV-8 strain re-emerged in France in 2015, triggering a second outbreak that is still ongoing. The origin of this virus and the mechanisms underlying its re-emergence are unknown. Here, we performed phylogenetic analyses of 164 whole BTV-8 genomes sampled throughout the two outbreaks. We demonstrate consistent clock-like virus evolution during both epizootics but found negligible evolutionary change between them. We estimate that the ancestor of the second outbreak dates from the height of the first outbreak in 2008. This implies that the virus had not been replicating for multiple years prior to its re-emergence in 2015. Given the absence of any known natural mechanism that could explain BTV-8 persistence over this long period without replication, we hypothesise that the second outbreak could have been initiated by accidental exposure of livestock to frozen material contaminated with virus from approximately 2008. Our work highlights new targets for pathogen surveillance programmes in livestock and illustrates the power of genomic epidemiology to identify pathways of infectious disease emergence. 
PY 2020
PD APR
SO Plos Biology
SN 1544-9173
PU Public Library Science
VL 18
IS 4
UT 000531804900020
DI 10.1371/journal.pbio.3000673
ID 77881
ER

EF