Contrasting selective patterns across the segmented genome of bluetongue virus in a global reassortment hotspot

Type Article
Date 2019-07
Language English
Author(s) Jacquot MaudeORCID1, 11, 12, Rao Pavuluri P.2, 13, Yadav Sarita3, 14, Nomikou KyriakiORCID4, Maan Sushila5, Jyothi Y. Krishna6, Reddy Narasimha7, Putty Kalyani7, Hemadri Divakar8, Singh Karam P.9, Maan Narender Singh5, Hegde Nagendra R.2, Mertens Peter3, 10, Biek RomanORCID1, 15
Affiliation(s) 1 : Univ Glasgow, Inst Biodivers Anim Hlth & Comparat Med, Boyd Orr Ctr Populat & Ecosyst Hlth, Coll Med Vet & Life Sci, Glasgow, Lanark, Scotland.
2 : Genome Valley Hyderabad, Ella Fdn, Hyderabad, Telangana, India.
3 : Pirbright Inst, Woking GU24 0NF, Surrey, England.
4 : Univ Glasgow, MRC, Ctr Virus Res, Glasgow, Lanark, Scotland.
5 : LLR Univ Vet & Anim Sci, Coll Vet Sci, Hisar, Haryana, India.
6 : Vet Biol & Res Inst, Vijayawada, Andhra Pradesh, India.
7 : PVNR Telangana Vet Univ, Hyderabad, Telangana, India.
8 : ICAR Natl Inst Vet Epidemiol & Dis Informat, Bengaluru, Karnataka, India.
9 : ICAR Indian Vet Res Inst, Ctr Anim Dis Res & Diag, Div Pathol, Bareilly 243122, Uttar Pradesh, India.
10 : Univ Nottingham, Sch Vet Med & Sci, Loughborough, Leics, England.
11 : Univ Brussels, Spatial Epidemiol Lab SpELL, Brussels, Belgium.
12 : INRA VetAgro Sup, UMR0346 Epidemiol Anim & Zoonot Dis, St Genes Champanelle, France.
13 : Biovet Private Ltd, Malur Taluk, Karnataka, India.
14 : ICAR Cent Inst Res Buffaloes, Hisar, Haryana, India.
15 : NIAB, Hyderabad, Telangana, India.
Source Virus Evolution (Oxford Univ Press), 2019-07 , Vol. 5 , N. 2 , P. vez027 (14p.)
DOI 10.1093/ve/vez027
WOS© Times Cited 6
Keyword(s) bluetongue virus, evolution, reassortment, India, selection
Abstract

For segmented viruses, rapid genomic and phenotypic changes can occur through the process of reassortment, whereby co-infecting strains exchange entire segments creating novel progeny virus genotypes. However, for many viruses with segmented genomes, this process and its effect on transmission dynamics remain poorly understood. Here, we assessed the consequences of reassortment for selection on viral diversity through time using bluetongue virus (BTV), a segmented arbovirus that is the causative agent of a major disease of ruminants. We analysed ninety-two BTV genomes isolated across four decades from India, where BTV diversity, and thus opportunities for reassortment, are among the highest in the world. Our results point to frequent reassortment and segment turnover, some of which appear to be driven by selective sweeps and serial hitchhiking. Particularly, we found evidence for a recent selective sweep affecting segment 5 and its encoded NS1 protein that has allowed a single variant to essentially invade the full range of BTV genomic backgrounds and serotypes currently circulating in India. In contrast, diversifying selection was found to play an important role in maintaining genetic diversity in genes encoding outer surface proteins involved in virus interactions (VP2 and VP5, encoded by segments 2 and 6, respectively). Our results support the role of reassortment in driving rapid phenotypic change in segmented viruses and generate testable hypotheses for in vitro experiments aiming at understanding the specific mechanisms underlying differences in fitness and selection across viral genomes.

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Jacquot Maude, Rao Pavuluri P., Yadav Sarita, Nomikou Kyriaki, Maan Sushila, Jyothi Y. Krishna, Reddy Narasimha, Putty Kalyani, Hemadri Divakar, Singh Karam P., Maan Narender Singh, Hegde Nagendra R., Mertens Peter, Biek Roman (2019). Contrasting selective patterns across the segmented genome of bluetongue virus in a global reassortment hotspot. Virus Evolution, 5(2), vez027 (14p.). Publisher's official version : https://doi.org/10.1093/ve/vez027 , Open Access version : https://archimer.ifremer.fr/doc/00667/77882/