FN Archimer Export Format
PT J
TI Comparative Population Genomics of the Borrelia burgdorferi Species Complex Reveals High Degree of Genetic Isolation among Species and Underscores Benefits and Constraints to Studying Intra-Specific Epidemiological Processes
BT 
AF JACQUOT, Maude
   GONNET, Mathieu
   FERQUEL, Elisabeth
   ABRIAL, David
   CLAUDE, Alexandre
   GASQUI, Patrick
   CHOUMET, Valerie
   CHARRAS-GARRIDO, Myriam
   GARNIER, Martine
   FAURE, Benjamin
   SERTOUR, Natacha
   DORR, Nelly
   DE GOER, Jocelyn
   VOURC'H, Gwenael
   BAILLY, Xavier
AS 1:1;2:1;3:2;4:1;5:1;6:1;7:2;8:1;9:2;10:1;11:2;12:1;13:1;14:1;15:1;
FF 1:;2:;3:;4:;5:;6:;7:;8:;9:;10:;11:;12:;13:;14:;15:;
C1 INRA, Epidemiol Anim UR346, F-63122 St Genes Champanelle, France.
   Inst Pasteur, CNR Borrelia, Paris, France.
C2 INRA, FRANCE
   INST PASTEUR, FRANCE
IN DOAJ
IF 3.234
TC 21
UR https://archimer.ifremer.fr/doc/00667/77887/80052.pdf
   https://archimer.ifremer.fr/doc/00667/77887/80053.tiff
   https://archimer.ifremer.fr/doc/00667/77887/80054.tiff
   https://archimer.ifremer.fr/doc/00667/77887/80055.tiff
   https://archimer.ifremer.fr/doc/00667/77887/80056.tiff
   https://archimer.ifremer.fr/doc/00667/77887/80057.doc
   https://archimer.ifremer.fr/doc/00667/77887/80058.doc
   https://archimer.ifremer.fr/doc/00667/77887/80059.doc
   https://archimer.ifremer.fr/doc/00667/77887/80060.doc
LA English
DT Article
AB Lyme borreliosis, one of the most frequently contracted zoonotic diseases in the Northern Hemisphere, is caused by bacteria belonging to different genetic groups within the Borrelia burgdorferi species complex, which are transmitted by ticks among various wildlife reservoirs, such as small mammals and birds. These features make the Borrelia burgdorferi species complex an attractive biological model that can be used to study the diversification and the epidemiology of endemic bacterial pathogens. We investigated the potential of population genomic approaches to study these processes. Sixty-three strains belonging to three species within the Borrelia burgdorferi complex were isolated from questing ticks in Alsace (France), a region where Lyme disease is highly endemic. We first aimed to characterize the degree of genetic isolation among the species sampled. Phylogenetic and coalescent-based analyses revealed clear delineations: there was a similar to 50 fold difference between intra-specific and inter-specific recombination rates. We then investigated whether the population genomic data contained information of epidemiological relevance. In phylogenies inferred using most of the genome, conspecific strains did not cluster in clades. These results raise questions about the relevance of different strategies when investigating pathogen epidemiology. For instance, here, both classical analytic approaches and phylodynamic simulations suggested that population sizes and migration rates were higher in B. garinii populations, which are normally associated with birds, than in B. burgdorferi s.s. populations. The phylogenetic analyses of the infection-related ospC gene and its flanking region provided additional support for this finding. Traces of recombination among the B. burgdorferi s. s. lineages and lineages associated with small mammals were found, suggesting that they shared the same hosts. Altogether, these results provide baseline evidence that can be used to formulate hypotheses regarding the host range of B. burgdorferi lineages based on population genomic data. 
PY 2014
PD APR
SO Plos One
SN 1932-6203
PU Public Library Science
VL 9
IS 4
UT 000336909100062
DI 10.1371/journal.pone.0094384
ID 77887
ER

EF