Differential expression of two ATPases revealed by lipid raft isolation from gills of euryhaline teleosts with different salinity preferences

Type Article
Date 2021-04
Language English
Author(s) Lin Yu-Ting1, 2, Hu Yau-Chung1, 2, Wang Yu-Chun3, Hsiao Man-Yun1, Lorin-Nebel Catherine4, Lee Tsung-Han1, 2
Affiliation(s) 1 : Department of Life Sciences, National Chung Hsing University, Taichung 402, Taiwan
2 : The iEGG and Animal Biotechnology Center, National Chung Hsing University, Taichung 402, Taiwan
3 : Planning and Information Division, Fisheries Research Institute, Keelung 202, Taiwan
4 : Univ. Montpellier, MARBEC (Univ. Montpellier, CNRS, IRD, IFREMER), 34095 Montpellier, France
Source Comparative Biochemistry And Physiology B-biochemistry & Molecular Biology (1096-4959) (Elsevier BV), 2021-04 , Vol. 253 , P. 110562 (8p.)
DOI 10.1016/j.cbpb.2021.110562
WOS© Times Cited 4
Keyword(s) Osmoregulation, Flotillin, Na+, K+-ATPase, V-type H+-ATPase, Euryhaline teleosts
Abstract

In euryhaline teleosts, Na+, K+-ATPase (NKA) and V-type H + -ATPase A (VHA A) are important ion-transporters located in cell membrane. Lipid rafts (LR) are plasma membrane microdomains enriched in cholesterol, sphingolipids, and proteins (e.g., flotillin). Flotillin is a LR-associated protein, commonly used as the LR marker. Previous mammalian studies showed that LR may play a crucial role in ion exchanges. Meanwhile, studies on mammals and rainbow trout showed that NKA were found to be present mainly in LR. However, little is known about LR in fish. Therefore, the present study aimed to investigate the involvement of branchial LR in osmoregulation of tilapia and milkfish, two euryhaline teleosts with different salinity preferences, by (i) extracting LR from the gills of euryhaline teleosts; (ii) detecting the abundance of LR marker protein (flotillin-2) and ion-transporters (NKA and VHA A) in branchial LR and non-LR of fresh water- and seawater-acclimated milkfish and tilapia. The results indicated that the protein abundance of LR marker, flotillin-2, changed with environmental salinities in branchial LR of tilapia. In addition, flotillin-2 and NKA were only found in LR in both tilapia and milkfish gills, while VHA A were mainly present in non-LR. Relative protein abundance of NKA was found to be significantly higher in gills of freshwater milkfish and seawater tilapia, while VHA A was significantly higher in gills of freshwater tilapia and milkfish. This study illustrated differential distribution and salinity-dependent expression of NKA and VHA A in cell membrane of gill tissues of euryhaline teleosts with different salinity preferences.

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Lin Yu-Ting, Hu Yau-Chung, Wang Yu-Chun, Hsiao Man-Yun, Lorin-Nebel Catherine, Lee Tsung-Han (2021). Differential expression of two ATPases revealed by lipid raft isolation from gills of euryhaline teleosts with different salinity preferences. Comparative Biochemistry And Physiology B-biochemistry & Molecular Biology, 253, 110562 (8p.). Publisher's official version : https://doi.org/10.1016/j.cbpb.2021.110562 , Open Access version : https://archimer.ifremer.fr/doc/00668/78058/