FN Archimer Export Format
PT J
TI Variable inter and intraspecies alkaline phosphatase activity within single cells of revived dinoflagellates
BT 
AF Girault, Mathias
   Siano, Raffaele
   Labry, Claire
   Latimier, Marie
   Jauzein, Cecile
   Beneyton, Thomas
   Buisson, Lionel
   Del Amo, Yolanda
   Baret, Jean-Christophe
AS 1:1;2:2;3:2;4:2;5:2;6:1;7:1;8:3;9:1,4;
FF 1:;2:PDG-ODE-DYNECO-PELAGOS;3:PDG-ODE-DYNECO-PELAGOS;4:PDG-ODE-DYNECO-PELAGOS;5:PDG-ODE-DYNECO-PELAGOS;6:;7:;8:;9:;
C1 Univ. Bordeaux, CNRS, CRPP, UMR5031, 33600, Pessac, France
   Ifremer, DYNECO, F-29280, Plouzané, France
   Université de Bordeaux, UMR CNRS 5805 EPOC, Station Marine d’Arcachon, 33120, Arcachon, France
   Institut Universitaire de France, 75005, Paris, France
C2 UNIV BORDEAUX, FRANCE
   IFREMER, FRANCE
   UNIV BORDEAUX, FRANCE
   INST UNIV FRANCE, FRANCE
SI BREST
SE PDG-ODE-DYNECO-PELAGOS
IF 9.18
TC 0
UR https://archimer.ifremer.fr/doc/00679/79097/81568.pdf
   https://archimer.ifremer.fr/doc/00679/79097/81569.pdf
LA English
DT Article
DE ;Climate-change ecology;Microbial ecology
AB Adaptation of cell populations to environmental changes is mediated by phenotypic variability at the single-cell level. Enzyme activity is a key factor in cell phenotype and the expression of the alkaline phosphatase activity (APA) is a fundamental phytoplankton strategy for maintaining growth under phosphate-limited conditions. Our aim was to compare the APA among cells and species revived from sediments of the Bay of Brest (Brittany, France), corresponding to a pre-eutrophication period (1940’s) and a beginning of a post-eutrophication period (1990’s) during which phosphate concentrations have undergone substantial variations. Both toxic marine dinoflagellate Alexandrium minutum and the non-toxic dinoflagellate Scrippsiella acuminata were revived from ancient sediments. Using microfluidics, we measured the kinetics of APA at the single-cell level. Our results indicate that all S. acuminata strains had significantly higher APA than A. minutum strains. For both species, the APA in the 1990’s decade was significantly lower than in the 1940’s. For the first time, our results reveal both inter and intraspecific variabilities of dinoflagellate APA and suggest that, at a half-century timescale, two different species of dinoflagellate may have undergone similar adaptative evolution to face environmental changes and acquire ecological advantages.   
PY 2021
SO The ISME Journal
SN 1751-7362
PU Springer Science and Business Media LLC
DI 10.1038/s41396-021-00904-2
ID 79097
ER

EF