FN Archimer Export Format
PT J
TI Allelochemicals of Alexandrium minutum: Kinetics of membrane disruption and photosynthesis inhibition in a co-occurring diatom
BT 
AF Long, Marc
   Peltekis, Alexandra
   González-Fernández, Carmen
   Hégaret, Helene
   Bailleul, Benjamin
AS 1:1,2;2:3;3:4;4:2;5:3;
FF 1:;2:;3:;4:;5:;
C1 School of Chemistry, University of Wollongong, NSW 2522, Australia
   Laboratoire des Sciences de l'Environnement Marin (LEMAR), UMR 6539 CNRS UBO IRD IFREMER –Institut Universitaire Européen de la Mer, Technopôle Brest-Iroise, Rue Dumont d'Urville, 29280 Plouzané, France
   Institut de Biologie Physico-Chimique, Laboratory of Chloroplast Biology and Light Sensing in Microalgae, UMR 7141, Centre National de la Recherche Scientifique (CNRS), Sorbonne université, 75005 Paris, France
   Immunobiotechnology for Aquaculture Group, Department of Cell Biology and Histology, Faculty of Biology, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, 30100 Murcia, Spain
C2 UNIV WOLLONGONG, AUSTRALIA
   CNRS, FRANCE
   CNRS, FRANCE
   UNIV MURCIA, SPAIN
UM LEMAR
IN WOS Cotutelle UMR
   copubli-france
   copubli-europe
   copubli-int-hors-europe
IF 5.905
TC 16
UR https://archimer.ifremer.fr/doc/00680/79191/81688.pdf
   https://archimer.ifremer.fr/doc/00680/79191/81689.docx
   https://archimer.ifremer.fr/doc/00680/79191/81690.xlsx
   https://archimer.ifremer.fr/doc/00680/79191/81691.jpg
   https://archimer.ifremer.fr/doc/00680/79191/81692.jpg
LA English
DT Article
DE ;Allelochemicals;Allelopathy;Alexandrium minutum;Chaetoceros muelleri;Photosynthesis;Membrane
AB Allelopathy is an efficient strategy by which some microalgae can outcompete other species. Allelochemicals from the toxic dinoflagellate Alexandrium minutum have deleterious effects on diatoms, inhibiting metabolism and photosynthesis and therefore give a competitive advantage to the dinoflagellate. The precise mechanisms of allelochemical interactions and the molecular target of allelochemicals remain however unknown. To understand the mechanisms, the short-term effects of A. minutum allelochemicals on the physiology of the diatom Chaetoceros muelleri were investigated. The effects of a culture filtrate were measured on the diatom cytoplasmic membrane integrity (polarity and permeability) using flow-cytometry and on the photosynthetic performance using fluorescence and absorption spectroscopy. Within 10 min, the unknown allelochemicals induced a depolarization of the cytoplasmic membranes and an impairment of photosynthesis through the inhibition of the plastoquinone-mediated electron transfer between photosystem II and cytochrome b6f. At longer time of exposure, the cytoplasmic membranes were permeable and the integrity of photosystems I, II and cytochrome b6f was compromised. Our demonstration of the essential role of membranes in this allelochemical interaction provides new insights for the elucidation of the nature of the allelochemicals. The relationship between cytoplasmic membranes and the inhibition of the photosynthetic electron transfer remains however unclear and warrants further investigation. 
PY 2021
PD MAR
SO Harmful Algae
SN 1568-9883
PU Elsevier BV
VL 103
UT 000663075400001
DI 10.1016/j.hal.2021.101997
ID 79191
ER

EF