FN Archimer Export Format
PT J
TI A MALDI-TOF MS database for fast identification of Vibrio spp. potentially pathogenic to marine mollusks
BT 
AF Moussa, M.
   Cauvin, E.
   Le Piouffle, A.
   Lucas, O.
   Bidault, Adeline
   Paillard, Christine
   Benoit, F.
   Thuillier, B.
   Treilles, M.
   Travers, Marie-Agnes
   Garcia, Celine
AS 1:1;2:2;3:3;4:4;5:5;6:5;7:2;8:3;9:4;10:1,6;11:1;
FF 1:;2:;3:;4:;5:;6:;7:;8:;9:;10:PDG-RBE-IHPE;11:PDG-RBE-SGMM-LGPMM;
C1 Ifremer, SG2M-LGPMM, Laboratoire de Génétique et Pathologie des Mollusques Marins, F-17390, La Tremblade, France
   Labeo-Manche, 1352 avenue de Paris, 50000, Saint-Lô, France
   Labocea, Avenue de la Plage des Gueux, 29330, Quimper, France
   Qualyse, ZI Montplaisir, 79220, Champdeniers Saint-Denis, France
   Univ Brest, CNRS, IRD, Ifremer, UMR6539 LEMAR, F-29280, Plouzané, France
   IHPE, Univ. Montpellier, CNRS, Ifremer, Univ. Perpignan Via Domitia, F-34090, Montpellier, France
C2 IFREMER, FRANCE
   LABEO, FRANCE
   LABOCEA, FRANCE
   QUALYSE, FRANCE
   UBO, FRANCE
   IFREMER, FRANCE
SI MONTPELLIER
   LA TREMBLADE
SE PDG-RBE-IHPE
   PDG-RBE-SGMM-LGPMM
UM LEMAR
   IHPE
IN WOS Ifremer UPR
   WOS Ifremer UMR
   WOS Cotutelle UMR
   copubli-france
   copubli-univ-france
IF 5.56
TC 17
UR https://archimer.ifremer.fr/doc/00680/79224/81732.pdf
   https://archimer.ifremer.fr/doc/00680/79224/81733.pdf
LA English
DT Article
DE ;Vibrio;MALDI-TOF MS;Database;Marine mollusk pathogens
AB In mollusk aquaculture, a large number of Vibrio species are considered major pathogens. Conventional methods based on DNA amplification and sequencing used to accurately identify Vibrio species are unsuitable for monitoring programs because they are time-consuming and expensive. The aim of this study was, therefore, to develop the MALDI-TOF MS method in order to establish a rapid identification technique for a large panel of Vibrio species. We created the EnviBase containing 120 main spectra projections (MSP) of the Vibrio species that are potentially responsible for mollusk diseases, comprising 25 species: V. aestuarianus, V. cortegadensis, V. tapetis and species belonging to the Coralliilyticus, Harveyi, Mediterranei, and Orientalis clades. Each MSP was constructed by the merger of raw spectra obtained from three different media and generated by three collaborating laboratories to increase the diversity of the conditions and thus obtain a good technique robustness. Perfect discrimination was obtained with all of the MSP created for the Vibrio species and even for very closely related species as V. europaeus and V. bivalvicida. The new EnviBase library was validated through a blind test on 100 Vibrio strains performed by our three collaborators who used the direct transfer and protein extraction methods. The majority of the Vibrio strains were successfully identified with the newly created EnviBase by the three laboratories for both protocol methods. This study documents the first development of a freely accessible database exclusively devoted to Vibrio found in marine environments, taking into account the high diversity of this genus. 
PY 2021
PD MAR
SO Applied Microbiology And Biotechnology
SN 0175-7598
PU Springer Science and Business Media LLC
VL 105
IS 6
UT 000618152700001
BP 2527
EP 2539
DI 10.1007/s00253-021-11141-0
ID 79224
ER

EF