FN Archimer Export Format
PT J
TI Passive Samplers, a Powerful Tool to Detect Viruses and Bacteria in Marine Coastal Areas
BT 
AF VINCENT HUBERT, Francoise
   Wacrenier, Candice
   Morga, Benjamin
   Lozach, Solen
   Quenot, Emmanuelle
   MEGE, Mickael
   Lecadet, Cyrielle
   Gourmelon, Michele
   Hervio-Heath, Dominique
   LE GUYADER, Soizick
AS 1:1;2:1;3:2;4:1;5:1;6:2;7:2;8:1;9:1;10:1;
FF 1:PDG-RBE-SGMM-LSEM;2:PDG-RBE-SGMM-LSEM;3:PDG-RBE-SGMM-LGPMM;4:PDG-RBE-SGMM;5:;6:PDG-RBE-SGMM-LGPMM;7:PDG-RBE-SGMM-LGPMM;8:PDG-RBE-SGMM;9:PDG-RBE-SGMM;10:PDG-RBE-SGMM-LSEM;
C1 Ifremer, Laboratoire de Microbiologie, LSEM/SG2M, Nantes, France
   Ifremer, Laboratoire de Génétique et Pathologie des Mollusques, LGPMM/SG2M, La Tremblade, France
C2 IFREMER, FRANCE
   IFREMER, FRANCE
SI NANTES
   LA TREMBLADE
   BREST
SE PDG-RBE-SGMM-LSEM
   PDG-RBE-SGMM-LGPMM
   PDG-RBE-SGMM
IN WOS Ifremer UPR
   DOAJ
IF 6.064
TC 11
UR https://archimer.ifremer.fr/doc/00682/79375/81893.pdf
   https://archimer.ifremer.fr/doc/00682/79375/81894.docx
   https://archimer.ifremer.fr/doc/00682/79375/81895.docx
   https://archimer.ifremer.fr/doc/00682/79375/81896.docx
   https://archimer.ifremer.fr/doc/00682/79375/81897.docx
   https://archimer.ifremer.fr/doc/00682/79375/81898.docx
   https://archimer.ifremer.fr/doc/00682/79375/81899.docx
   https://archimer.ifremer.fr/doc/00682/79375/81900.docx
   https://archimer.ifremer.fr/doc/00682/79375/81901.docx
LA English
DT Article
DE ;norovirus;Ostreid herpes virus 1 μvar;Vibrio spp;microbial source tracking;sea;passive sampler;oyster (Crassostrea gigas)
AB The detection of viruses and bacteria which can pose a threat either to shellfish health or shellfish consumers remains difficult. The current detection methods rely on point sampling of water, a method that gives a snapshot of the microorganisms present at the time of sampling. In order to obtain better representativeness of the presence of these microorganisms over time, we have developed passive sampling using the adsorption capacities of polymer membranes. Our objectives here were to assess the feasibility of this methodology for field detection. Different types of membrane were deployed in coastal waters over 2 years and the microorganisms tested using qPCR were: human norovirus (NoV) genogroups (G)I and II, sapovirus, Vibrio spp. and the species Vibrio alginolyticus, V. cholerae, V. vulnificus, and V. parahaemolyticus, OsHV-1 virus, and bacterial markers of fecal contamination. NoV GII, Vibrio spp., and the AllBac general Bacteroidales marker were quantified on the three types of membrane. NoV GII and OsHV-1 viruses followed a seasonal distribution. All membranes were favorable for NoV GII detection, while Zetapor was more adapted for OsHV-1 detection. Nylon was more adapted for detection of Vibrio spp. and the AllBac marker. The quantities of NoV GII, AllBac, and Vibrio spp. recovered on membranes increased with the duration of exposure. This first application of passive sampling in seawater is particularly promising in terms of an early warning system for the prevention of contamination in oyster farming areas and to improve our knowledge on the timing and frequency of disease occurence. 
PY 2021
PD FEB
SO Frontiers In Microbiology
SN 1664-302X
PU Frontiers Media SA
VL 12
UT 000626285900001
DI 10.3389/fmicb.2021.631174
ID 79375
ER

EF