FN Archimer Export Format
PT J
TI Hemocyte siRNA uptake is increased by 5′ cholesterol-TEG addition in   Biomphalaria glabrata   , snail vector of schistosome
BT 
AF Portet, Anaïs
   Galinier, Richard
   Lassalle, Damien
   Faille, Alexandre
   Gourbal, Benjamin
   Duval, David
AS 1:1,2;2:1;3:1;4:3,4,5;5:1;6:1;
FF 1:;2:;3:;4:;5:;6:;
C1 IHPE UMR 5244, CNRS, IFREMER, University of Montpellier, University of Perpignan, Perpignan, France
   Department of Medicine, Molecular Immunity Unit, University of Cambridge, Cambridge, United Kingdom
   Department of Haematology, University of Cambridge, Cambridge, United Kingdom
   Wellcome Trust-Medical Research Council Stem Cell Institute, University of Cambridge, Cambridge, United Kingdom
   Cambridge Institute for Medical Research, Cambridge, United Kingdom
C2 UNIV MONTPELLIER, FRANCE
   UNIV CAMBRIDGE, UK
   UNIV CAMBRIDGE, UK
   UNIV CAMBRIDGE, UK
   CIMR, UK
UM IHPE
IN WOS Cotutelle UMR
   DOAJ
   copubli-europe
IF 3.061
TC 6
UR https://archimer.ifremer.fr/doc/00682/79376/81902.pdf
   https://archimer.ifremer.fr/doc/00682/79376/81903.png
   https://archimer.ifremer.fr/doc/00682/79376/81904.xlsx
   https://archimer.ifremer.fr/doc/00682/79376/81905.png
LA English
DT Article
DE ;Biomphalaria glabrata;Cholesteryl TEG;RNAi interference;BgTEP1;Hemocyte
AB Biomphalaria glabrata is one of the snail intermediate hosts of Schistosoma mansoni, the causative agent of intestinal schistosomiasis disease. Numerous molecular studies using comparative approaches between susceptible and resistant snails to S. mansoni infection have helped identify numerous snail key candidates supporting such susceptible/resistant status. The functional approach using RNA interference (RNAi) remains crucial to validate the function of such candidates. CRISPR-Cas systems are still under development in many laboratories, and RNA interference remains the best tool to study B. glabrata snail genetics. Herein, we describe the use of modified small interfering RNA (siRNA) molecules to enhance cell delivery, especially into hemocytes, the snail immune cells. Modification of siRNA with 5′ Cholesteryl TriEthylene Glycol (Chol-TEG) promotes cellular uptake by hemocytes, nearly eightfold over that of unmodified siRNA. FACS analysis reveals that more than 50% of hemocytes have internalized Chol-TEG siRNA conjugated to Cy3 fluorophores, 2 hours only after in vivo injection into snails. Chol-TEG siRNA targeting BgTEP1 (ThioEster-containing Protein), a parasite binding protein, reduced BgTEP1 transcript expression by 70–80% compared to control. The level of BgTEP1 protein secreted in the hemolymph was also decreased. However, despite the BgTEP1 knock-down at both RNA and protein levels, snail compatibility with its sympatric parasite is not affected suggesting functional redundancy among the BgTEP genes family in snail-schistosoma interaction. 
PY 2021
PD FEB
SO Peerj
SN 2167-8359
PU PeerJ
VL 9
UT 000620772400011
DI 10.7717/peerj.10895
ID 79376
ER

EF