FN Archimer Export Format PT J TI Can shellfish be used to monitor SARS-CoV-2 in the coastal environment? BT AF Desdouits, Marion Piquet, Jean-Come Wacrenier, Candice Le Mennec, Cecile Parnaudeau, Sylvain Jousse, Sarah Rocq, Sophie Bigault, Lionel Contrant, Maud Garry, Pascal Chavanon, Fabienne Gabellec, Raoul Lamort, Laure Lebrun, Luc LE GALL, Patrik Meteigner, Claire Schmitt, Anne Seugnet, Jean-Luc Serais, Ophelie Peltier, Cécile Bressolette-Bodin, Céline Blanchard, Yannick LE GUYADER, Soizick AS 1:1;2:1;3:1;4:1;5:1;6:1;7:1;8:2;9:2;10:1;11:3;12:4;13:5;14:6;15:7;16:11;17:4;18:8;19:9;20:10;21:10;22:2;23:1; FF 1:PDG-RBE-SGMM-LSEM;2:PDG-RBE-SGMM-LSEM;3:PDG-RBE-SGMM-LSEM;4:PDG-RBE-SGMM-LSEM;5:PDG-RBE-SGMM-LSEM;6:PDG-RBE-SGMM-LSEM;7:PDG-RBE-SGMM-LSEM;8:;9:;10:PDG-RBE-SGMM-LSEM;11:PDG-ODE-LITTORAL-LERPAC;12:PDG-ODE-LITTORAL-LERMPL;13:PDG-ODE-LITTORAL-LERN;14:PDG-ODE-LITTORAL-LERBO;15:PDG-ODE-LITTORAL-LERBN;16:PDG-ODE-LITTORAL-LERAR;17:PDG-ODE-LITTORAL-LERMPL;18:PDG-ODE-LITTORAL-LERPC;19:PDG-ODE-LITTORAL-LERLR;20:;21:;22:;23:PDG-RBE-SGMM-LSEM; C1 Ifremer, laboratoire de Microbiologie, SG2M/LSEM, BP 21105, 44311 Nantes, France ANSES, Génétique Virale et Biosécurité, Ploufragan, France Ifremer, Laboratoire Environnement Ressource Provence-Azur-Corse, la Seyne sur Mer, France Ifremer, Laboratoire Environnement Ressource Morbihan Pays de la Loire, Lorient, France Ifremer, Laboratoire Environnement Ressource Normandie, Port en Bessin, France Ifremer, Laboratoire Environnement Ressource Bretagne Occidentale, Concarneau, France Ifremer, Laboratoire Environnement Ressource Bretagne Nord, Dinard, France Ifremer, Laboratoire Environnement Ressource Pertuis-Charentais, la Tremblade, France Ifremer, Laboratoire Environnement Ressource Languedoc Roussillon, Sète, France Nantes Université, Centre de Recherche en Transplantation et Immunologie, UMR 1064, ITUN, 44000 Nantes, France Ifremer, Laboratoire Environnement Ressource Arcachon, Arcachon, France C2 IFREMER, FRANCE ANSES, FRANCE IFREMER, FRANCE IFREMER, FRANCE IFREMER, FRANCE IFREMER, FRANCE IFREMER, FRANCE IFREMER, FRANCE IFREMER, FRANCE UNIV NANTES, FRANCE IFREMER, FRANCE SI NANTES TOULON LORIENT PORT-EN-BESSIN CONCARNEAU DINARD ARCACHON LA TREMBLADE SETE SE PDG-RBE-SGMM-LSEM PDG-ODE-LITTORAL-LERPAC PDG-ODE-LITTORAL-LERMPL PDG-ODE-LITTORAL-LERN PDG-ODE-LITTORAL-LERBO PDG-ODE-LITTORAL-LERBN PDG-ODE-LITTORAL-LERAR PDG-ODE-LITTORAL-LERPC PDG-ODE-LITTORAL-LERLR UM MARBEC IN WOS Ifremer UPR WOS Ifremer UMR copubli-france copubli-univ-france IF 10.754 TC 23 UR https://archimer.ifremer.fr/doc/00684/79569/86750.pdf LA English DT Article DE ;SARS-CoV-2;Coastal environment;Seawater;Shellfish;Detection method;Genomic detection AB The emergence and worldwide spread of SARS-CoV-2 raises new concerns and challenges regarding possible environmental contamination by this virus through spillover of human sewage, where it has been detected. The coastal environment, under increasing anthropogenic pressure, is subjected to contamination by a large number of human viruses from sewage, most of them being non-enveloped viruses like norovirus. When reaching coastal waters, they can be bio-accumulated by filter-feeding shellfish species such as oysters. Methods to detect this viral contamination were set up for the detection of non-enveloped enteric viruses, and may need optimization to accommodate enveloped viruses like coronaviruses (CoV). Here, we aimed at assessing methods for the detection of CoV, including SARS-CoV-2, in the coastal environment and testing the possibility that SARS-CoV-2 can contaminate oysters, to monitor the contamination of French shores by SARS-CoV-2 using both seawater and shellfish. Using the porcine epidemic diarrhea virus (PEDV), a CoV, as surrogate for SARS-CoV-2, and Tulane virus, as surrogate for non-enveloped viruses such as norovirus, we assessed and selected methods to detect CoV in seawater and shellfish. Seawater-based methods showed variable and low yields for PEDV. In shellfish, the current norm for norovirus detection was applicable to CoV detection. Both PEDV and heat-inactivated SARS-CoV-2 could contaminate oysters in laboratory settings, with a lower efficiency than a calicivirus used as control. Finally, we applied our methods to seawater and shellfish samples collected from April to August 2020 in France, where we could detect the presence of human norovirus, a marker of human fecal contamination, but not SARS-CoV-2. Together, our results validate methods for the detection of CoV in the coastal environment, including the use of shellfish as sentinels of the microbial quality of their environment, and suggest that SARS-CoV-2 did not contaminate the French shores during the summer season. PY 2021 PD JUN SO Science Of The Total Environment SN 0048-9697 PU Elsevier BV VL 778 UT 000655712300003 DI 10.1016/j.scitotenv.2021.146270 ID 79569 ER EF