FN Archimer Export Format
PT J
TI Fatty acid isotopic fractionation in the diatom Chaetoceros muelleri
BT 
AF Remize, Marine
   Planchon, Frederic
   Loh, Ai Ning
   Le Grand, Fabienne
   Mathieu-Resuge, Margaux
   Bideau, Antoine
   Corvaisier, Rudolph
   Volety, Aswani
   Soudant, Philippe
AS 1:1,2;2:1;3:2;4:5;5:1,3;6:1;7:5;8:4;9:5;
FF 1:;2:;3:;4:;5:;6:;7:;8:;9:;
C1 Univ Brest, CNRS, IRD, Ifremer, UMR 6539 LEMAR, F-29280 Plouzané, France
   University of North Carolina Wilmington, Department of Earth and Ocean Sciences, Center for Marine Science, 5600 Marvin K. Moss Ln, Wilmington, NC 28403, USA
   WasserCluster Lunz – Inter-University Centre for Aquatic Ecosystem Research, Dr. Carl Kupelwieser Promenade 5, A-3293 Lunz am See, Austria
   Elon University, 50 Campus Drive, Elon, NC 27244, USA
   Univ Brest, CNRS, IRD, Ifremer, UMR 6539 LEMAR, F-29280 Plouzané, France
C2 UBO, FRANCE
   UNIV N CAROLINA, USA
   WASSERCLUSTER LUNZ, AUSTRIA
   UNIV ELON, USA
   CNRS, FRANCE
UM LEMAR
IN WOS Cotutelle UMR
   copubli-europe
   copubli-int-hors-europe
IF 5.276
TC 2
UR https://archimer.ifremer.fr/doc/00684/79570/82303.pdf
LA English
DT Article
DE ;Fractionation;Stable isotopes;Photosynthesis;Phytoplankton;Chaetoceros muelleri;Fatty acid synthesis
AB Carbon isotopic fractionation was studied during the development of the diatom Chaetoceros muelleri grown in batch culture with 13C-depleted CO2 addition. Cellular and growth parameters and isotopic composition of dissolved inorganic carbon and particulate organic carbon were monitored every two days, while the content and isotopic composition of individual fatty acid in polar lipid and neutral lipid were measured on the 5th day (end of exponential phase), 10th and 14th days (stationary phase). Continuous addition of petrochemical CO2 to the algae led to a rapid and strong modification of dissolved inorganic carbon isotopic composition with cascading effects on particulate organic carbon and fatty acid isotopic compositions. Carbon isotope fractionation in Chaetoceros muelleri ranged from 17‰ to 25‰ and changed according to culture ages. Isotopic fractionation into fatty acids, overall, was similar between polar and neutral lipids, and was systematically higher than in particulate organic carbon. At the end of the exponential growth phase, the isotopic composition of individual fatty acids varied from −51.3‰ to −58.4‰. At this culture age, large differences in the isotopic compositions between fatty acids were observed. Polyunsaturated fatty acids such as 16:3n-4, 18:4n-3, and 20:5n-3 were more strongly 13C-depleted than other fatty acids such as 14:0, 16:0, 16:1n-7 or 18:1n-9. These results showed how isotopic effects occur during the desaturation and elongation phases. Such isotopic effects were also supported by the lower δ13C of averaged δ13C of saturated fatty acids and monounsaturated fatty acids as compared to those of polyunsaturated fatty acids. However, during the stationary phase, fatty acid isotopic compositions were less variable and closer to particulate organic carbon, while saturated and monounsaturated fatty acids were more depleted than polyunsaturated fatty acids. Our study underlined the importance of consideration of phytoplankton physiological status when conducting ecological and biogeochemical studies as they appeared to strongly control phytoplankton carbon isotopic composition. 
PY 2021
PD APR
SO Algal Research-biomass Biofuels And Bioproducts
SN 2211-9264
PU Elsevier BV
VL 54
UT 000632338300004
DI 10.1016/j.algal.2020.102164
ID 79570
ER

EF