FN Archimer Export Format
PT J
TI Cell free Microcystis aeruginosa spent medium affects Daphnia magna survival and stress response
BT 
AF Bojadzija Savic, Gorenka
   Colinet, Hervé
   Bormans, Myriam
   Edwards, Christine
   Lawton, Linda A.
   Briand, Enora
   Wiegand, Claudia
AS 1:1;2:1;3:1;4:2;5:2;6:3;7:1;
FF 1:;2:;3:;4:;5:;6:PDG-ODE-DYNECO-PHYC;7:;
C1 UMR ECOBIO 6553 CNRS, Université de Rennes 1, Campus de Beaulieu, Rennes, France
   School of Pharmacy and Life Sciences, Robert Gordon University, Aberdeen, United Kingdom
   IFREMER, Phycotoxins Laboratory, F-44311 Nantes, France
C2 UNIV RENNES, FRANCE
   UNIV ABERDEEN, UK
   IFREMER, FRANCE
SI NANTES
SE PDG-ODE-DYNECO-PHYC
IN WOS Ifremer UPR
   copubli-france
   copubli-europe
   copubli-univ-france
IF 3.035
TC 3
UR https://archimer.ifremer.fr/doc/00684/79572/82239.pdf
LA English
DT Article
DE ;Zooplankton;Cyanobacteria;Secondary metabolites;PCC7806;Oxidative stress;Transcriptomics
AB Primary consumers in freshwater ecosystems, such as the zooplankton organism Daphnia magna, are highly affected by cyanobacteria, both as they may use it as a food source but also by cyanobacterial metabolites present in the water. Here, we investigate the impacts of cyanobacterial metabolites focussing on the environmental realistic scenario of the naturally released mixture without crushing cyanobacterial cells or their uptake as food. Therefore, D. magna were exposed to two concentrations of cell free cyanobacterial spent medium from Microcystis aeruginosa PCC 7806 to represent higher and lower ecologically-relevant concentrations of cyanobacterial metabolites. Including microcystin-LR, 11 metabolites have been detected of which 5 were quantified. Hypothesising concentration and time dependent negative impact, survival, gene expression marking digestion and metabolism, oxidative stress response, cell cycle and molting as well as activities of detoxification and antioxidant enzymes were followed for 7 days. D. magna suffered from oxidative stress as both catalase and glutathione S-transferase enzyme activities significantly decreased, suggesting enzyme exhaustibility after 3 and 7 days. Moreover, gene-expressions of the 4 stress markers (glutathione S-transferase, glutathione peroxidase, catalase and thioredoxin) were merely downregulated after 7 days of exposure. Energy allocation (expression of Glyceraldehyde-3-phosphate dehydrogenase) was increased after 3 days but decreased as well after 7 days exposure. Cell cycle was impacted time dependently but differently by the two concentrations, along with an increasing downregulation of myosin heavy chain responsible for cell arrangement and muscular movements. Deregulation of nuclear hormone receptor genes indicate that D. magna hormonal steering including molting seemed impaired despite no detection of microviridin J in the extracts. As a consequence of all those responses and presumably of more than investigated molecular and physiological changes, D. magna survival was impaired over time, in a concentration dependent manner. Our results confirm that besides microcystin-LR, other secondary metabolites contribute to negative impact on D. magna survival and stress response. 
PY 2021
PD MAY
SO Toxicon
SN 0041-0101
PU Elsevier BV
VL 195
UT 000637220900007
BP 37
EP 47
DI 10.1016/j.toxicon.2021.03.009
ID 79572
ER

EF