FN Archimer Export Format
PT J
TI Antiproliferative Properties of Scandium Exopolysaccharide Complexes on Several Cancer Cell Lines
BT 
AF Muñoz-Garcia, Javier
   Mazza, Mattia
   Alliot, Cyrille
   Sinquin, Corinne
   Colliec-Jouault, Sylvia
   Heymann, Dominique
   Huclier-Markai, Sandrine
AS 1:1;2:2,3;3:2,4;4:5;5:5;6:1,6;7:2,3;
FF 1:;2:;3:;4:PDG-RBE-BRM-LEMMMB;5:PDG-RBE-BRM-LEMMMB;6:;7:;
C1 Institut de Cancérologie de l’Ouest, Université de Nantes, Blvd Jacques Monod, F-44805 Saint-Herblain, France
   GIP ARRONAX, 1 rue Aronnax, CEDEX 3, F-44817 Nantes, France
   Laboratoire SUBATECH, 4 rue Alfred Kastler, BP 20722, CEDEX 3, F-44307 Nantes, France
   Centre de Recherche en Cancérologie et Immunologie Nantes Angers, INSERM, U892, 8 quai Moncousu, CEDEX 1, F-44007 Nantes, France
   IFREMER, Institut Français de Recherche pour L’exploitation de la mer, rue de l’Ile d’Yeu, BP21105, CEDEX 3, F-44311 Nantes, France
   Department of Oncology and Metabolism, Medical School, University of Sheffield, Sheffield S10 2TN, UK
C2 UNIV NANTES, FRANCE
   GIP ARRONAX, FRANCE
   SUBATECH, FRANCE
   INSERM, FRANCE
   IFREMER, FRANCE
   UNIV SHEFFIELD, UK
SI NANTES
SE PDG-RBE-BRM-LEMMMB
IN WOS Ifremer UPR
   DOAJ
   copubli-france
   copubli-europe
   copubli-univ-france
IF 6.085
TC 5
UR https://archimer.ifremer.fr/doc/00686/79826/82630.pdf
LA English
DT Article
DE ;exopolysaccharides;scandium;theranostic;cancer cell lines;proliferation
AB Antimetastatic properties on both murine and human osteosarcoma cell lines (POS-1 and KHOS) have been evidenced using exopolysaccharide (EPS) derivatives, produced by Alteromonas infernus bacterium. These derivatives had no significant effect on the cell cycle neither a pro-apoptotic effect on osteosarcoma cells. Based on this observation, these EPSs could be employed as new drug delivery systems for therapeutic uses. A theranostic approach, i.e., combination of a predictive biomarker with a therapeutic agent, has been developed notably by combining with true pair of theranostic radionuclides, such as scandium 47Sc/44Sc. However, it is crucial to ensure that, once complexation is done, the biological properties of the vector remain intact, allowing the molecular tropism of the ligand to recognize its molecular target. It is important to assess if the biological properties of EPS evidenced on osteosarcoma cell lines remain when scandium is complexed to the polymers and can be extended to other cancer cell types. Scandium-EPS complexes were thus tested in vitro on human cell lines: MNNG/HOS osteosarcoma, A375 melanoma, A549 lung adenocarcinoma, U251 glioma, MDA231 breast cancer, and Caco2 colon cancer cells. An xCELLigence Real Cell Time Analysis (RTCA) technology assay was used to monitor for 160 h, the proliferation kinetics of the different cell lines. The tested complexes exhibited an anti-proliferative effect, this effect was more effective compared to EPS alone. This increase of the antiproliferative properties was explained by a change in conformation of EPS complexes due to their polyelectrolyte nature that was induced by complexation. Alterations of both growth factor-receptor signaling, and transmembrane protein interactions could be the principal cause of the antiproliferative effect. These results are very promising and reveal that EPS can be coupled to scandium for improving its biological effects and also suggesting that no major structural modification occurs on the ligand 
PY 2021
PD MAR
SO Marine Drugs
SN 1660-3397
PU MDPI AG
VL 19
IS 3
UT 000633858500001
DI 10.3390/md19030174
ID 79826
ER

EF