TY - JOUR T1 - Optimal proteome allocation and the temperature dependence of microbial growth laws A1 - Mairet,Francis A1 - Gouzé,Jean-Luc A1 - de Jong,Hidde AD - Ifremer, Physiology and Biotechnology of Algae laboratory, Nantes, France AD - Université Côte d’Azur, Inria, INRAE, CNRS, Sorbonne Université, Biocore team, Sophia Antipolis, France AD - Université Grenoble Alpes, Inria, Grenoble, France UR - https://archimer.ifremer.fr/doc/00686/79829/ DO - 10.1038/s41540-021-00172-y N2 - Although the effect of temperature on microbial growth has been widely studied, the role of proteome allocation in bringing about temperature-induced changes remains elusive. To tackle this problem, we propose a coarse-grained model of microbial growth, including the processes of temperature-sensitive protein unfolding and chaperone-assisted (re)folding. We determine the proteome sector allocation that maximizes balanced growth rate as a function of nutrient limitation and temperature. Calibrated with quantitative proteomic data for Escherichia coli, the model allows us to clarify general principles of temperature-dependent proteome allocation and formulate generalized growth laws. The same activation energy for metabolic enzymes and ribosomes leads to an Arrhenius increase in growth rate at constant proteome composition over a large range of temperatures, whereas at extreme temperatures resources are diverted away from growth to chaperone-mediated stress responses. Our approach points at risks and possible remedies for the use of ribosome content to characterize complex ecosystems with temperature variation. Y1 - 2021/03 PB - Springer Science and Business Media LLC JF - Npj Systems Biology And Applications SN - 2056-7189 VL - 7 IS - 1 ID - 79829 ER -