FN Archimer Export Format
PT J
TI Optimal proteome allocation and the temperature dependence of microbial growth laws
BT 
AF Mairet, Francis
   Gouzé, Jean-Luc
   de Jong, Hidde
AS 1:1;2:2;3:3;
FF 1:PDG-RBE-BRM-LPBA;2:;3:;
C1 Ifremer, Physiology and Biotechnology of Algae laboratory, Nantes, France
   Université Côte d’Azur, Inria, INRAE, CNRS, Sorbonne Université, Biocore team, Sophia Antipolis, France
   Université Grenoble Alpes, Inria, Grenoble, France
C2 IFREMER, FRANCE
   INRIA, FRANCE
   UNIV GRENOBLE ALPES, FRANCE
SI NANTES
SE PDG-RBE-BRM-LPBA
IN WOS Ifremer UPR
   DOAJ
   copubli-france
   copubli-univ-france
IF 4.671
TC 13
UR https://archimer.ifremer.fr/doc/00686/79829/82633.pdf
   https://archimer.ifremer.fr/doc/00686/79829/82634.pdf
   https://archimer.ifremer.fr/doc/00686/79829/82635.pdf
LA English
DT Article
AB Although the effect of temperature on microbial growth has been widely studied, the role of proteome allocation in bringing about temperature-induced changes remains elusive. To tackle this problem, we propose a coarse-grained model of microbial growth, including the processes of temperature-sensitive protein unfolding and chaperone-assisted (re)folding. We determine the proteome sector allocation that maximizes balanced growth rate as a function of nutrient limitation and temperature. Calibrated with quantitative proteomic data for Escherichia coli, the model allows us to clarify general principles of temperature-dependent proteome allocation and formulate generalized growth laws. The same activation energy for metabolic enzymes and ribosomes leads to an Arrhenius increase in growth rate at constant proteome composition over a large range of temperatures, whereas at extreme temperatures resources are diverted away from growth to chaperone-mediated stress responses. Our approach points at risks and possible remedies for the use of ribosome content to characterize complex ecosystems with temperature variation. 
PY 2021
PD MAR
SO Npj Systems Biology And Applications
SN 2056-7189
PU Springer Science and Business Media LLC
VL 7
IS 1
UT 000627259400001
DI 10.1038/s41540-021-00172-y
ID 79829
ER

EF