Application of a Genus-Specific LAMP Assay for Schistosome Species to Detect Schistosoma haematobium x Schistosoma bovis Hybrids

Type Article
Date 2021-03
Language English
Author(s) Crego-Vicente Beatriz1, Fernández-Soto PedroORCID1, Febrer-Sendra Begoña1, García-Bernalt Diego JuanORCID1, Boissier Jérôme2, Angora Etienne K.3, 4, 5, Oleaga Ana6, Muro AntonioORCID1
Affiliation(s) 1 : Infectious and Tropical Diseases Research Group (e-INTRO), Research Centre for Tropical Diseases at the University of Salamanca (IBSAL-CIETUS), Biomedical Research Institute of Salamanca, Faculty of Pharmacy, University of Salamanca, 37007 Salamanca, Spain
2 : IHPE, Université Montpellier, CNRS, Ifremer, Université Perpignan Via Domitia, 66100 Perpignan, France
3 : Swiss Tropical and Public Health Institute, P.O. Box CH-4002 Basel, Switzerland
4 : Department of Public Health, University of Basel, P.O. Box CH-4003 Basel, Switzerland
5 : Unité de Formation et de Recherche Sciences Pharmaceutiques et Biologiques, Université Félix Houphouët-Boigny, Abidjan BPV 34, Côte d’Ivoire
6 : Parasitology Laboratory, Institute of Natural Resources and Agrobiology (IRNASA, CSIC), Cordel de Merinas 40-52, 37008 Salamanca, Spain
Source Journal Of Clinical Medicine (2077-0383) (MDPI AG), 2021-03 , Vol. 10 , N. 6 , P. 1308 (13p.)
DOI 10.3390/jcm10061308
WOS© Times Cited 1
Note This article belongs to the Special Issue Epidemiology, Immunology, and Control of Schistosomiasis
Keyword(s) LAMP, schistosomiasis, schistosome hybrids, Schistosoma haematobium, Schistosoma bovis, molecular diagnosis, species-specific LAMP, genus-specific LAMP

Schistosomiasis is a disease of great medical and veterinary importance in tropical and subtropical regions caused by different species of parasitic flatworms of the genus Schistosoma. The emergence of natural hybrids of schistosomes indicate the risk of possible infection to humans and their zoonotic potential, specifically for Schistosoma haematobium and S. bovis. Hybrid schistosomes have the potential to replace existing species, generate new resistances, pathologies and extending host ranges. Hybrids may also confuse the serological, molecular and parasitological diagnosis. Currently, LAMP technology based on detection of nucleic acids is used for detection of many agents, including schistosomes. Here, we evaluate our previously developed species-specific LAMP assays for S. haematobium, S. mansoni, S. bovis and also the genus-specific LAMP for the simultaneous detection of several Schistosoma species against both DNA from pure and, for the first time, S. haematobium x S. bovis hybrids. Proper operation was evaluated with DNA from hybrid schistosomes and with human urine samples artificially contaminated with parasites’ DNA. LAMP was performed with and without prior DNA extraction. The genus-specific LAMP properly amplified pure Schistosoma species and different S. haematobium-S. bovis hybrids with different sensitivity. The Schistosoma spp.-LAMP method is potentially adaptable for field diagnosis and disease surveillance in schistosomiasis endemic areas where human infections by schistosome hybrids are increasingly common

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Crego-Vicente Beatriz, Fernández-Soto Pedro, Febrer-Sendra Begoña, García-Bernalt Diego Juan, Boissier Jérôme, Angora Etienne K., Oleaga Ana, Muro Antonio (2021). Application of a Genus-Specific LAMP Assay for Schistosome Species to Detect Schistosoma haematobium x Schistosoma bovis Hybrids. Journal Of Clinical Medicine, 10(6), 1308 (13p.). Publisher's official version : , Open Access version :