TY - JOUR T1 - Application of a Genus-Specific LAMP Assay for Schistosome Species to Detect Schistosoma haematobium x Schistosoma bovis Hybrids A1 - Crego-Vicente,Beatriz A1 - Fernández-Soto,Pedro A1 - Febrer-Sendra,Begoña A1 - García-Bernalt Diego,Juan A1 - Boissier,Jérôme A1 - Angora,Etienne K. A1 - Oleaga,Ana A1 - Muro,Antonio AD - Infectious and Tropical Diseases Research Group (e-INTRO), Research Centre for Tropical Diseases at the University of Salamanca (IBSAL-CIETUS), Biomedical Research Institute of Salamanca, Faculty of Pharmacy, University of Salamanca, 37007 Salamanca, Spain AD - IHPE, Université Montpellier, CNRS, Ifremer, Université Perpignan Via Domitia, 66100 Perpignan, France AD - Swiss Tropical and Public Health Institute, P.O. Box CH-4002 Basel, Switzerland AD - Department of Public Health, University of Basel, P.O. Box CH-4003 Basel, Switzerland AD - Unité de Formation et de Recherche Sciences Pharmaceutiques et Biologiques, Université Félix Houphouët-Boigny, Abidjan BPV 34, Côte d’Ivoire AD - Parasitology Laboratory, Institute of Natural Resources and Agrobiology (IRNASA, CSIC), Cordel de Merinas 40-52, 37008 Salamanca, Spain UR - https://archimer.ifremer.fr/doc/00686/79840/ DO - 10.3390/jcm10061308 KW - LAMP KW - schistosomiasis KW - schistosome hybrids KW - Schistosoma haematobium KW - Schistosoma bovis KW - molecular diagnosis KW - species-specific LAMP KW - genus-specific LAMP N2 - Schistosomiasis is a disease of great medical and veterinary importance in tropical and subtropical regions caused by different species of parasitic flatworms of the genus Schistosoma. The emergence of natural hybrids of schistosomes indicate the risk of possible infection to humans and their zoonotic potential, specifically for Schistosoma haematobium and S. bovis. Hybrid schistosomes have the potential to replace existing species, generate new resistances, pathologies and extending host ranges. Hybrids may also confuse the serological, molecular and parasitological diagnosis. Currently, LAMP technology based on detection of nucleic acids is used for detection of many agents, including schistosomes. Here, we evaluate our previously developed species-specific LAMP assays for S. haematobium, S. mansoni, S. bovis and also the genus-specific LAMP for the simultaneous detection of several Schistosoma species against both DNA from pure and, for the first time, S. haematobium x S. bovis hybrids. Proper operation was evaluated with DNA from hybrid schistosomes and with human urine samples artificially contaminated with parasites’ DNA. LAMP was performed with and without prior DNA extraction. The genus-specific LAMP properly amplified pure Schistosoma species and different S. haematobium-S. bovis hybrids with different sensitivity. The Schistosoma spp.-LAMP method is potentially adaptable for field diagnosis and disease surveillance in schistosomiasis endemic areas where human infections by schistosome hybrids are increasingly common Y1 - 2021/03 PB - MDPI AG JF - Journal Of Clinical Medicine SN - 2077-0383 VL - 10 IS - 6 ID - 79840 ER -