FN Archimer Export Format
PT J
TI Application of a Genus-Specific LAMP Assay for Schistosome Species to Detect Schistosoma haematobium x Schistosoma bovis Hybrids
BT 
AF Crego-Vicente, Beatriz
   Fernández-Soto, Pedro
   Febrer-Sendra, Begoña
   García-Bernalt Diego, Juan
   Boissier, Jérôme
   Angora, Etienne K.
   Oleaga, Ana
   Muro, Antonio
AS 1:1;2:1;3:1;4:1;5:2;6:3,4,5;7:6;8:1;
FF 1:;2:;3:;4:;5:;6:;7:;8:;
C1 Infectious and Tropical Diseases Research Group (e-INTRO), Research Centre for Tropical Diseases at the University of Salamanca (IBSAL-CIETUS), Biomedical Research Institute of Salamanca, Faculty of Pharmacy, University of Salamanca, 37007 Salamanca, Spain
   IHPE, Université Montpellier, CNRS, Ifremer, Université Perpignan Via Domitia, 66100 Perpignan, France
   Swiss Tropical and Public Health Institute, P.O. Box CH-4002 Basel, Switzerland
   Department of Public Health, University of Basel, P.O. Box CH-4003 Basel, Switzerland
   Unité de Formation et de Recherche Sciences Pharmaceutiques et Biologiques, Université Félix Houphouët-Boigny, Abidjan BPV 34, Côte d’Ivoire
   Parasitology Laboratory, Institute of Natural Resources and Agrobiology (IRNASA, CSIC), Cordel de Merinas 40-52, 37008 Salamanca, Spain
C2 UNIV SALAMANCA, SPAIN
   UNIV MONTPELLIER, FRANCE
   SWISS TPH, SWITZERLAND
   UNIV BASEL, SWITZERLAND
   UNIV FELIX HOUPHOUET BOIGNY, COTE IVOIRE
   CSIC, SPAIN
UM IHPE
IN WOS Cotutelle UMR
   DOAJ
   copubli-europe
   copubli-int-hors-europe
   copubli-sud
IF 4.964
TC 2
UR https://archimer.ifremer.fr/doc/00686/79840/82659.pdf
LA English
DT Article
DE ;LAMP;schistosomiasis;schistosome hybrids;Schistosoma haematobium;Schistosoma bovis;molecular diagnosis;species-specific LAMP;genus-specific LAMP
AB Schistosomiasis is a disease of great medical and veterinary importance in tropical and subtropical regions caused by different species of parasitic flatworms of the genus Schistosoma. The emergence of natural hybrids of schistosomes indicate the risk of possible infection to humans and their zoonotic potential, specifically for Schistosoma haematobium and S. bovis. Hybrid schistosomes have the potential to replace existing species, generate new resistances, pathologies and extending host ranges. Hybrids may also confuse the serological, molecular and parasitological diagnosis. Currently, LAMP technology based on detection of nucleic acids is used for detection of many agents, including schistosomes. Here, we evaluate our previously developed species-specific LAMP assays for S. haematobium, S. mansoni, S. bovis and also the genus-specific LAMP for the simultaneous detection of several Schistosoma species against both DNA from pure and, for the first time, S. haematobium x S. bovis hybrids. Proper operation was evaluated with DNA from hybrid schistosomes and with human urine samples artificially contaminated with parasites’ DNA. LAMP was performed with and without prior DNA extraction. The genus-specific LAMP properly amplified pure Schistosoma species and different S. haematobium-S. bovis hybrids with different sensitivity. The Schistosoma spp.-LAMP method is potentially adaptable for field diagnosis and disease surveillance in schistosomiasis endemic areas where human infections by schistosome hybrids are increasingly common 
PY 2021
PD MAR
SO Journal Of Clinical Medicine
SN 2077-0383
PU MDPI AG
VL 10
IS 6
UT 000651930500001
DI 10.3390/jcm10061308
ID 79840
ER

EF