FN Archimer Export Format
PT J
TI Induction of Phlorotannins and Gene Expression in the Brown Macroalga Fucus vesiculosus in Response to the Herbivore Littorina littorea
BT 
AF Emeline, Creis Bendelac
   Ludovic, Delage
   Laurent, Vallet
   Catherine, Leblanc
   Kruse, Inken
   Erwan, Ar Gall
   Florian, Weinberger
   Philippe, Potin
AS 1:1,2;2:1;3:1;4:1;5:3;6:4;7:3;8:1;
FF 1:;2:;3:;4:;5:;6:;7:;8:;
C1 Integrative Biology of Marine Models (LBI2M), CNRS, Sorbonne Université, UMR 8227, Station Biologique, Place Georges Teissier, 29680 Roscoff, Brittany, France
   International Research Laboratory IRL 3614, CNRS, Sorbonne Université, PUC, UACH, Evolutionary Biology and Ecology of Algae, EBEA, Station Biologique, 29680 Roscoff, Brittany, France
   Helmholtz Centre for Ocean Research (GEOMAR), Düsternbrooker Weg 20, 24105 Kiel, Germany
   Laboratoire des Sciences de l’Environnement Marin, UBO European Institute for Marine Studies IUEM, University of Brest—Western Brittany, UMR 6539 LEMAR, Technopôle Brest Iroise, Rue Dumont d’Urville, 29280 Plouzané, Brittany, France
C2 CNRS, FRANCE
   CNRS, FRANCE
   IFM GEOMAR, GERMANY
   UBO, FRANCE
UM LEMAR
IN WOS Cotutelle UMR
   DOAJ
   copubli-france
   copubli-europe
IF 6.085
TC 9
UR https://archimer.ifremer.fr/doc/00688/79981/82940.pdf
   https://archimer.ifremer.fr/doc/00688/79981/82941.pdf
LA English
DT Article
DE ;phlorotannins;inducible defense;gene expression;grazing;Fucus vesiculosus
AB Mechanisms related to the induction of phlorotannin biosynthesis in marine brown algae remain poorly known. Several studies undertaken on fucoid species have shown that phlorotannins accumulate in the algae for several days or weeks after being exposed to grazing, and this is measured by direct quantification of soluble phenolic compounds. In order to investigate earlier inducible responses involved in phlorotannin metabolism, Fucus vesiculosus was studied between 6 and 72 h of grazing by the sea snail Littorina littorea. In this study, the quantification of soluble phenolic compounds was complemented by a Quantitative real-time PCR (qRT-PCR) approach applied on genes that are potentially involved in either the phlorotannin metabolism or stress responses. Soluble phlorotannin levels remained stable during the kinetics and increased significantly only after 12 h in the presence of grazers, compared to the control, before decreasing to the initial steady state for the rest of the kinetics. Under grazing conditions, the expression of vbpo, cyp450 and ast6 genes was upregulated, respectively, at 6 h, 12 h and 24 h, and cyp450 gene was downregulated after 72 h. Interestingly, the pksIII gene involved in the synthesis of phloroglucinol was overexpressed under grazing conditions after 24 h and 72 h. This study supports the hypothesis that phlorotannins are able to provide an inducible chemical defense under grazing activity, which is regulated at different stages of the stress response. 
PY 2021
PD APR
SO Marine Drugs
SN 1660-3397
PU MDPI AG
VL 19
IS 4
UT 000643192700001
DI 10.3390/md19040185
ID 79981
ER

EF