Implication of the Type IV Secretion System in the Pathogenicity of Vibrio tapetis, the Etiological Agent of Brown Ring Disease Affecting the Manila Clam Ruditapes philippinarum

Type Article
Date 2021-04
Language English
Author(s) Rahmani Alexandra1, Delavat François2, 4, Lambert Christophe1, Le Goic Nelly1, Dabas Eric3, Paillard Christine1, Pichereau Vianney4
Affiliation(s) 1 : Univ Brest, CNRS, IRD, Ifremer, UMR 6539 LEMAR, Plouzane, France
2 : UMR CNRS 6286 UFIP, University of Nantes, Nantes, France
3 : Univ Brest, CNRS, IRD, Ifremer, UMR 6539 LEMAR, Plouzane, France
4 : Univ Brest, CNRS, IRD, Ifremer, UMR 6539 LEMAR, Plouzane, France
Source Frontiers In Cellular And Infection Microbiology (2235-2988) (Frontiers Media SA), 2021-04 , Vol. 11 , P. 634427 (13p.)
DOI 10.3389/fcimb.2021.634427
WOS© Times Cited 1
Keyword(s) Brown Ring Disease, Vibrio tapetis, Ruditapes philippinarum, hemocytes, type four secretion system, rounding phenotype, colonization, mutagenesis
Abstract

Vibrio tapetis is a Gram-negative bacterium that causes infections of mollusk bivalves and fish. The Brown Ring Disease (BRD) is an infection caused by V. tapetis that primarily affects the Manila clam Ruditapes philippinarum. Recent studies have shown that a type IV secretion system (T4SS) gene cluster is exclusively found in strains of V. tapetis pathogenic to clams. However, whether the T4SS is implicated or not during the infection process remains unknown. The aim of this study was to create and characterize a V. tapetis T4SS null mutant, obtained by a near-complete deletion of the virB4 gene, in order to determine the role of T4SS in the development of BRD. This study demonstrated that the T4SS is neither responsible for the loss of hemocyte adhesion capacities, nor for the decrease of the lysosomal activity during BRD. Nevertheless, we observed a 50% decrease of the BRD prevalence and a decrease of mortality dynamics with the ΔvirB4 mutant. This work demonstrates that the T4SS of V. tapetis plays an important role in the development of BRD in the Manila clam.

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Publisher's official version 13 954 KB Open access
Supplementary Figure 1 | PCR with primers 170513-170514 that amplified virB4 gene. A fragment of 173 bp is expected if the gene virB4 is present, no amplification is expected if the gene virB4 is ... 11 KB Open access
Supplementary Figure 2 | Cytograms and Fluorescence Peaks corresponding to results of count of non-adherent hemocytes (laser FITC, Figure 3) and amount of acidic organelles (laser APC, Figure 4) in .. 55 KB Open access
Supplementary Figure 3 | Picture of a Brown Ring Disease deposit on an infected Manila clam of the experiment described in Figure 5. (A) 1 valve pictured without a zoom. (B, C) Picture realized with a 20 KB Open access
Table S1 2 304 KB Open access
Table S2 13 KB Open access
Table S3 13 KB Open access
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How to cite 

Rahmani Alexandra, Delavat François, Lambert Christophe, Le Goic Nelly, Dabas Eric, Paillard Christine, Pichereau Vianney (2021). Implication of the Type IV Secretion System in the Pathogenicity of Vibrio tapetis, the Etiological Agent of Brown Ring Disease Affecting the Manila Clam Ruditapes philippinarum. Frontiers In Cellular And Infection Microbiology, 11, 634427 (13p.). Publisher's official version : https://doi.org/10.3389/fcimb.2021.634427 , Open Access version : https://archimer.ifremer.fr/doc/00692/80383/