FN Archimer Export Format PT J TI Implication of the Type IV Secretion System in the Pathogenicity of Vibrio tapetis, the Etiological Agent of Brown Ring Disease Affecting the Manila Clam Ruditapes philippinarum BT AF Rahmani, Alexandra Delavat, François Lambert, Christophe Le Goic, Nelly Dabas, Eric Paillard, Christine Pichereau, Vianney AS 1:1;2:2,4;3:1;4:1;5:3;6:1;7:4; FF 1:;2:;3:;4:;5:;6:;7:; C1 Univ Brest, CNRS, IRD, Ifremer, UMR 6539 LEMAR, Plouzane, France UMR CNRS 6286 UFIP, University of Nantes, Nantes, France Univ Brest, CNRS, IRD, Ifremer, UMR 6539 LEMAR, Plouzane, France Univ Brest, CNRS, IRD, Ifremer, UMR 6539 LEMAR, Plouzane, France C2 CNRS, FRANCE UNIV NANTES, FRANCE IRD, FRANCE UBO, FRANCE UM LEMAR IN WOS Cotutelle UMR DOAJ copubli-france copubli-univ-france IF 6.073 TC 4 UR https://archimer.ifremer.fr/doc/00692/80383/83507.pdf https://archimer.ifremer.fr/doc/00692/80383/83508.jpeg https://archimer.ifremer.fr/doc/00692/80383/83509.jpeg https://archimer.ifremer.fr/doc/00692/80383/83510.jpeg https://archimer.ifremer.fr/doc/00692/80383/83511.pdf https://archimer.ifremer.fr/doc/00692/80383/83512.docx https://archimer.ifremer.fr/doc/00692/80383/83513.docx LA English DT Article DE ;Brown Ring Disease;Vibrio tapetis;Ruditapes philippinarum;hemocytes;type four secretion system;rounding phenotype;colonization;mutagenesis AB Vibrio tapetis is a Gram-negative bacterium that causes infections of mollusk bivalves and fish. The Brown Ring Disease (BRD) is an infection caused by V. tapetis that primarily affects the Manila clam Ruditapes philippinarum. Recent studies have shown that a type IV secretion system (T4SS) gene cluster is exclusively found in strains of V. tapetis pathogenic to clams. However, whether the T4SS is implicated or not during the infection process remains unknown. The aim of this study was to create and characterize a V. tapetis T4SS null mutant, obtained by a near-complete deletion of the virB4 gene, in order to determine the role of T4SS in the development of BRD. This study demonstrated that the T4SS is neither responsible for the loss of hemocyte adhesion capacities, nor for the decrease of the lysosomal activity during BRD. Nevertheless, we observed a 50% decrease of the BRD prevalence and a decrease of mortality dynamics with the ΔvirB4 mutant. This work demonstrates that the T4SS of V. tapetis plays an important role in the development of BRD in the Manila clam. PY 2021 PD APR SO Frontiers In Cellular And Infection Microbiology SN 2235-2988 PU Frontiers Media SA VL 11 UT 000649655200001 DI 10.3389/fcimb.2021.634427 ID 80383 ER EF