FN Archimer Export Format
PT J
TI Experimental Infection of the Biomphalaria glabrata Vector Snail by Schistosoma mansoni Parasites Drives Snail Microbiota Dysbiosis
BT 
AF Portet, Anaïs
   Toulza, Eve
   Lokmer, Ana
   Huot, Camille
   Duval, David
   Galinier, Richard
   Gourbal, Benjamin
AS 1:1;2:1;3:2;4:1;5:1;6:1;7:1;
FF 1:;2:;3:;4:;5:;6:;7:;
C1 IHPE, University Montpellier, CNRS, Ifremer, University Perpignan Via Domitia, 66860 Perpignan, France
   Laboratory of Eco-Anthropology UMR 7206 CNRS-MNHN-Paris 7, 75005 Paris, France
C2 UNIV PERPIGNAN, FRANCE
   MNHN, FRANCE
UM IHPE
IN WOS Cotutelle UMR
   DOAJ
   copubli-france
IF 4.926
TC 17
UR https://archimer.ifremer.fr/doc/00696/80784/84121.pdf
   https://archimer.ifremer.fr/doc/00696/80784/84122.zip
LA English
DT Article
DE ;microbiota;bacteria;Biomphalaria snail;Schistosoma infection;immune response;dysbiosis
AB Host-parasite interaction can result in a strong alteration of the host-associated microbiota. This dysbiosis can affect the fitness of the host; can modify pathogen interaction and the outcome of diseases. Biomphalaria glabrata is the snail intermediate host of the trematode Schistosoma mansoni, the agent of human schistosomiasis, causing hundreds of thousands of deaths every year. Here, we present the first study of the snail bacterial microbiota in response to Schistosoma infection. We examined the interplay between B. glabrata, S. mansoni and host microbiota. Snails were infected and the microbiota composition was analysed by 16S rDNA amplicon sequencing approach. We demonstrated that the microbial composition of water did not affect the microbiota composition. Then, we characterised the Biomphalaria bacterial microbiota at the individual scale in both naive and infected snails. Sympatric and allopatric strains of parasites were used for infections and re-infections to analyse the modification or dysbiosis of snail microbiota in different host-parasite co-evolutionary contexts. Concomitantly, using RNAseq, we investigated the link between bacterial microbiota dysbiosis and snail anti-microbial peptide immune response. This work paves the way for a better understanding of snail/schistosome interaction and should have critical consequences in terms of snail control strategies for fighting schistosomiasis disease in the field. 
PY 2021
PD MAY
SO Microorganisms
SN 2076-2607
PU MDPI AG
VL 9
IS 5
UT 000662370000001
DI 10.3390/microorganisms9051084
ID 80784
ER

EF